Dataset Overview
RBPLARP4
Knock-MethodsiRNA
RBP FamilyLa
Biosample NameHEK293T
Tissue TypeKidney
Biosample TypeCell line
# of Control3
# of Treat3
SpeciesHuman
Data SourceGEO
Profile IDGSE139548
PlatformGPL18573
Pubmed ID-

LARP4 - Target Gene Network

up-regulated gene   
down-regulated gene   
Gene Type: # of Top Genes:

Differential Expression Genes responding to KD/KO of LARP4
Gene Mean Expr. of Control Mean Expr. of KD/KO logFC PValue FDR

Function Analysis of Target Genes of LARP4


Differential Alternative Splicing
  • Exon Skipping
  • Mutually Exclusive Exons
  • Alternative 5’ splice site
  • Alternative 3’ splice site
  • Intron Retention
RBP LongES_LongEE_ShortES_ShortEE_FlankingES_FlankingEE pValue △Inc Dataset ID Sashimi plot
RBP LongES_LongEE_ShortES_ShortEE_FlankingES_FlankingEE pValue △Inc Dataset ID Sashimi plot
RBP LongES_LongEE_ShortES_ShortEE_FlankingES_FlankingEE pValue △Inc Dataset ID Sashimi plot
RBP LongES_LongEE_ShortES_ShortEE_FlankingES_FlankingEE pValue △Inc Dataset ID Sashimi plot
RBP LongES_LongEE_ShortES_ShortEE_FlankingES_FlankingEE pValue △Inc Dataset ID Sashimi plot

Differential Variants in RNA (DVRs)
Chrom Site Ref_allele Alt_allele Pvalue Genename Strand KnownRNAediting RepeatName RepeatFamily
chr131732602CA0.00336SNRNP40-FALSE
chr291843429GA0.00037LOC654342-FALSE
chr1207942535AG0.00102CD46+TRUEAluSg4SINE,Alu
chr340577810AG0.00896ZNF621+TRUEAluSzSINE,Alu
chr636894338AG0.00607C6orf89+FALSEGA-richSINE,Alu
chr1244572643CA0.00560ADSS-FALSE
chr2206987725TC0.00108.FALSEAluJbSINE,Alu
chr145452227GC0.00279EIF2B3-FALSE
chr51574319TA0.00158SDHAP3-FALSE
chr638140505AG0.00109BTBD9-FALSE
chr125570549CA0.00387RSRP1-FALSE
chr2170607000TA0.00590PHOSPHO2-KLHL23,KLHL23+FALSE
chr1226172221TC0.00118SDE2-FALSEL1MEfSINE,Alu
chr791742142CA0.00478CYP51A1-FALSEAluSx3SINE,Alu
chr1780899281GA0.00053TBCD+FALSE
chr1773703945CT0.00242SAP30BP+FALSEMIRcSINE,Alu
chr1120612006GA0.00602NOTCH2-FALSE
chrX123047170AG0.00855XIAP+FALSEAluSxSINE,Alu
chr116939857CT0.00091NBPF1-FALSE
chr1533064148TC1.59288E-05FMN1-FALSE
chr154691910AG0.00481SSBP3-FALSE
chr3156259229TC1.62025E-06SSR3-TRUEAluSq2SINE,Alu
chr386993604GT0.00253VGLL3-FALSEAluJoSINE,Alu
chr1175161849GT0.00030KIAA0040-FALSE
chr348787219AG0.00024PRKAR2A-FALSE
chr126755303AG0.00129LIN28A+TRUEAluSx1SINE,Alu
chr2242512138AG9.62871E-05BOK+FALSE
chr167874731TC0.00145SERBP1-TRUEAluSx3SINE,Alu
chr116921146TC0.00382NBPF1-FALSE
chr986584220GA0.00318HNRNPK-FALSE
chr631379931GA0.00093.FALSE
chr110520378TC0.00115.FALSEAluSx1SINE,Alu
chr236776054CT0.00117CRIM1+FALSE
chr1327407AG0.00752.FALSE
chr1145689611CA0.00142NBPF20,NBPF10+FALSEAluSgSINE,Alu
chr2204301760CT0.00313RAPH1-FALSE
chr2101635538CT0.00069TBC1D8,RPL31+,-FALSECharlie17SINE,Alu
chr1453242746TC0.00051GNPNAT1-TRUEAluJrSINE,Alu
chr184373309TC0.00627TTLL7-FALSE
chr126608016GA1.44245E-06.FALSE
chr2178406763TC0.00452AGPS+FALSE
chr3100083423TC0.00678TOMM70-FALSEFAMSINE,Alu
chr8144357975CT0.00444GLI4+FALSE
chr3100467676AT0.00288TFG+FALSE
chr164114301TC0.00955PGM1+FALSE
chr1203277252GA0.00559BTG2+FALSE
chr549692280TC9.73531E-05EMB-FALSEL2cSINE,Alu
chr1120695049GA0.00129.FALSE
chr133071096AG0.00692ZBTB8A+TRUEAluSx1SINE,Alu
chr170611296AT0.00061LRRC40-FALSE
chr1186281696TG0.00564PRG4,TPR+,-FALSE
chr552393421CT0.00144MOCS2-FALSEL1MA9SINE,Alu
chr177166017GA0.00146.FALSE
chr12125269475TC0.00184SCARB1-FALSE
chr1179829093TC0.00056TOR1AIP2-TRUEAluJrSINE,Alu
chr167874849TC0.00169SERBP1-TRUEAluSxSINE,Alu
chr162516683GC0.00966PATJ+FALSE
chr194032944CT0.00147BCAR3-FALSE
chr110521103TC0.00788DFFA-TRUEAluYSINE,Alu
chr138463504CT0.00068FHL3-FALSE
chr826435509TC0.00121DPYSL2+FALSE(TC)nSINE,Alu
chr358306797AG8.72021E-05.FALSEAluSzSINE,Alu
chr1013320123CA0.00830PHYH-FALSE
chr570503889CA0.00131.FALSE
chr124151858CT0.00104HMGCL-FALSE
chr19329105TA0.00233H6PD+FALSE
chr1179326295AG0.00035SOAT1+TRUE
chr126449340GA0.00711PDIK1L+FALSE
chr3137787192TC0.00764DZIP1L-FALSE
chr210179657AC0.00035.FALSEL1ME4bSINE,Alu
chr1109746825AG0.00094KIAA1324+TRUEAluJbSINE,Alu
chr7149318375CT0.00383ZNF767P-FALSE
chr2206987136TC0.00189.FALSEAluSxSINE,Alu
chr255516938AT0.00265CCDC88A-FALSE(GA)nSINE,Alu
chr624720534TC0.00053.FALSE
chr315296359CT2.99463E-05SH3BP5,SH3BP5-AS1+,-FALSE
chr110520532TC0.00011.FALSEAluSx1SINE,Alu
chr1114940227TC0.00852TRIM33-FALSE(T)nSINE,Alu
chr127992825GA0.00192IFI6-FALSE
chr577655330TC0.00050SCAMP1-AS1-TRUEAluSx4SINE,Alu
chr123636274GT0.00165HNRNPR-FALSE
chr1220323448CT0.00223RAB3GAP2-FALSE
chr132508534AG0.00666KHDRBS1+FALSE
chr1144614842CG0.00015NBPF20+FALSE
chr7123390058AG0.00102.FALSE
chr110520099TC6.30717E-05.FALSEAluSxSINE,Alu
chr1910602447CT0.00878KEAP1-FALSE
chr1229631734TC0.00611NUP133-FALSE
chr116641899CT0.00213FBXO42-FALSE
chr275888160AC0.00368MRPL19+FALSEL1MB4SINE,Alu
chr122418122TC0.00790CDC42+FALSE
chr237330435TC3.14905E-06.FALSEAluSq2SINE,Alu
chr185785550CA0.00156DDAH1-FALSE
chr1145376020GA0.00067NBPF20,NBPF10+FALSE
chr3156258308TC0.00323SSR3-TRUEAluYk3SINE,Alu
chr41387223AG0.00155CRIPAK+TRUEAluSxSINE,Alu
chr1109817245CT2.70011E-05CELSR2+FALSE
chr119543456TC3.77297E-05LOC101927895,EMC1+,-FALSEAluJbSINE,Alu
chr438945689CT1.01800E-05FAM114A1+FALSEAluScSINE,Alu
chr1113672616AG0.00553.FALSEAluJbSINE,Alu
chr1089004545TC0.00057NUTM2A-AS1-FALSEMSTASINE,Alu
chr127250642AG0.00020NUDC+FALSE
chr3194373833GA0.00217LSG1-FALSE
chr1246829616TC0.00139CNST+FALSE
chr5149361415AG0.00290SLC26A2+TRUE
chr5125962528AG0.00924PHAX+TRUEAluSzSINE,Alu
chr1207942427AG0.00822CD46+TRUEAluSg4SINE,Alu
chr141487492CG0.00221SLFNL1-AS1+FALSE
chr117734207GA0.00020RCC2-FALSE
chr110520072TC0.00529.FALSEAluSxSINE,Alu
chr537290400TC0.00085.FALSEAluSzSINE,Alu
chr1110300588CT0.00232EPS8L3-FALSE
chr189525966TC0.00039GBP1-FALSE
chr1204374665GA0.00717PPP1R15B-FALSE
chr413602231GA0.00646BOD1L1-FALSE
chr1109818160TG0.00745CELSR2+FALSE
chr299812446AG0.00461MRPL30+TRUEAluSxSINE,Alu
chr119953130TA0.00595MINOS1,MINOS1-NBL1+FALSE
chr1911560483AG0.00775PRKCSH+TRUEAluSxSINE,Alu
chr11252662TC0.00136CPSF3L-TRUEAluSpSINE,Alu
chr642855954AG3.50340E-05RPL7L1+TRUEAluSzSINE,Alu
chr1109816293GC0.00759CELSR2+FALSE
chr119543352TC0.00086LOC101927895,EMC1+,-FALSEAluJbSINE,Alu
chr626095468AG0.00980HFE+TRUEAluYSINE,Alu
chr5170832637CT0.00071NPM1+FALSE
chr2171850174TG3.41223E-05TLK1-FALSE
chr117298140CG0.00584CROCC+FALSE
chr110520888CT0.00960DFFA-FALSE
chr189648329TC0.00093GBP4-TRUEAluYg6SINE,Alu
chr146126142GC0.00171GPBP1L1-FALSE
chr174849959GT0.00636PFN1-FALSE
chr121793735CG0.00405NBPF3+FALSE
chr110519888TC0.00218.FALSEAluJrSINE,Alu
chr312624070CT2.78764E-07MKRN2+FALSE
chr237331565TC0.00121.FALSEAluJbSINE,Alu
chr1116238478CT0.00834VANGL1+FALSE
chr193308166CT0.00334FAM69A-FALSE
chr110042955AG0.00430NMNAT1+TRUEAluScSINE,Alu
chr237429737AG0.00549CEBPZOS,CEBPZ+,-FALSEAluSzSINE,Alu
chr1988503AT0.00402AGRN+FALSE
chr1145512166TA0.00736NBPF20,NBPF10,RBM8A,GNRHR2+,-FALSEAluYSINE,Alu
chr28996701GT0.00063MBOAT2-FALSEAluSzSINE,Alu
chr611185161CT0.00297NEDD9-FALSE
chr1717425631CT0.00036PEMT-FALSE
chr285546320GT0.00749TGOLN2-FALSEAluScSINE,Alu
chr110520258TC0.00396.FALSEAluSxSINE,Alu
chr1119427687GC0.00823TBX15-FALSE
chr42940147TC0.00036NOP14-AS1,NOP14+,-FALSEAluSpSINE,Alu
chr2218571345AG0.00113PEX26+TRUEAluSzSINE,Alu
chr717384395AG0.00856AHR+TRUEAluSxSINE,Alu
chr1116609833GA0.00012SLC22A15+FALSE
chr1201438097GC2.75480E-05PHLDA3-FALSE
chr255459824CT0.00786RPS27A+FALSE
chr4152025795TA0.00516RPS3A+FALSE
chr286249906TC0.00352LOC90784-TRUEAluJrSINE,Alu
chr933039686GA0.00542DNAJA1+FALSE
chr16283348TC0.00247ICMT-TRUEAluSx3SINE,Alu
chr349452957AT0.00066TCTA+FALSEAluSgSINE,Alu
chr13729587AG0.00580CEP104-FALSE(AT)nSINE,Alu
chr128825331AG0.00051PHACTR4+TRUEAluSgSINE,Alu
chr1469704553GT7.62587E-05EXD2+FALSE
chr1134370TC0.00327.FALSEAluSgSINE,Alu
chr1153609268GA0.00263CHTOP+FALSE
chr1205738302GA0.00661RAB29-FALSE
chr914764CT0.00491WASH1-FALSE
chr1100490275AG0.00748SLC35A3+TRUEAluSc5SINE,Alu
chr127212513TC0.00260GPN2-FALSE
chr1184762524TC0.00471FAM129A-TRUEAluSzSINE,Alu
chr159142713TC0.00246MYSM1-FALSE
chr11344998AG0.00062.FALSEAluSgSINE,Alu
chr3101542436AG0.00023NXPE3+TRUEAluJrSINE,Alu
chr642856978AG0.00819RPL7L1+TRUEAluSpSINE,Alu
chr136067752TC5.48772E-07PSMB2-TRUEAluSzSINE,Alu
chr1911257634TC0.00823SPC24-TRUEAluSpSINE,Alu
chr116932845TG0.00732NBPF1-FALSEL1MC2SINE,Alu
chr3112320485TC0.00107LINC01279-TRUEAluJbSINE,Alu
chr1256510697GA0.00989RPL41+FALSE
chr116889050AC0.00669NBPF1-FALSE
chr1161931628GC0.00476ATF6+FALSE
chr1541593269AG0.00196.FALSEAluSz6SINE,Alu
chr111347154AT0.00366UBIAD1+FALSE
chr119934880TC0.00456MINOS1,MINOS1-NBL1,RPS14P3+FALSE
chr5133918133AC0.00413JADE2+FALSE
chr187213174TG0.00081SH3GLB1+FALSE
chr224223256AG0.00711UBXN2A+TRUEAluScSINE,Alu
chr121766762CG0.00259NBPF3+FALSE
chr1728506279GA0.00634NSRP1+FALSE
chr255462102AG0.00026RPS27A+FALSE
chr1145512950AG2.03454E-08NBPF20,NBPF10,RBM8A,GNRHR2+,-FALSEAluSz6SINE,Alu
chr825272425CT0.00605DOCK5+FALSE
chr1071993094TG0.00078PPA1-FALSE
chr12133298275AG4.13546E-09PGAM5+TRUEAluSpSINE,Alu
chr128835934AG0.00566SNHG3,RCC1+TRUEAluSx4SINE,Alu
chr1541597574AG2.24597E-05.FALSEAluSz6SINE,Alu
chr238971642GC0.00013SRSF7-FALSE
chr135648842GA0.00015SFPQ-FALSEAluSx3SINE,Alu
chr921331486TG0.00675KLHL9-FALSE
chr310192747AG0.00065VHL+TRUEAluSx1SINE,Alu
chr337366459CA2.94197E-05GOLGA4+FALSE
chr1648386354AG0.00064LONP2,MIR5095+TRUEAluSz6SINE,Alu
chr1109192051TC0.00124HENMT1-TRUEAluJrSINE,Alu
chr115988702TC0.00362.FALSE
chr1475202402AG0.00141FCF1+TRUEAluScSINE,Alu
chr3156259288TC0.00072SSR3-TRUEAluSq2SINE,Alu
chr236704035CA0.00012CRIM1+FALSE
chr310192596AG9.40892E-06VHL+TRUEAluSx1SINE,Alu
chr455147769CT0.00079PDGFRA+FALSE
chr19162348TC0.00824.FALSEAluSxSINE,Alu
chr2232319578CA0.00844NCL-FALSEA-richSINE,Alu
chr344791062TC0.00035KIAA1143-FALSE
chr133147947AG0.00345SYNC,RBBP4+,-FALSEAluSxSINE,Alu
chr333909025AT0.00796PDCD6IP+FALSE
chr136067760TC1.59237E-06PSMB2-TRUEAluSzSINE,Alu
chr147717189AG0.00384STIL-FALSE
chr1145285310CA5.09397E-05NBPF20,NOTCH2NL+FALSE
chr1016554732TA0.00706PTER+FALSE
chr16582176TC0.00694NOL9-TRUEAluSq4SINE,Alu
chr340577745AG0.00044ZNF621+TRUEAluSzSINE,Alu
chr29661424GC0.00130ADAM17-FALSE
chr1205056476TC0.00096RBBP5-FALSEAluSxSINE,Alu
chr154691922TA0.00219SSBP3-FALSE
chr7100271437CA0.00026GNB2+FALSE
chr237328034TC0.00035.FALSE
chr133149496AG0.00662SYNC,RBBP4+,-FALSEAluSx1SINE,Alu
chr152520866CT0.00195TXNDC12-FALSE
chr110520725TC0.00234DFFA-TRUEAluSc8SINE,Alu
chr127107669TA0.00636ARID1A+FALSE(A)nSINE,Alu
chr1847918818AG3.50049E-05SKA1+TRUEAluJbSINE,Alu
chr2220037666AG0.00796CNPPD1-FALSE
chr1220232138TC0.00011RNU5F-1,BPNT1+,-FALSEAluSzSINE,Alu
chr119586501AG0.00288MRTO4+TRUEAluSzSINE,Alu
chr1761502458TA0.00317TANC2+FALSE
chr3113528895AT0.00191ATP6V1A+FALSEAluSpSINE,Alu
chr1773940888TC2.69053E-06ACOX1-TRUEAluSx1SINE,Alu
chr63154871AC7.50591E-06TUBB2A-FALSE
chr272968438GT0.00147EXOC6B-FALSE
chr3183889337AT3.46641E-05DVL3+FALSE
chr3189713205TC4.97145E-05P3H2-FALSE
chr1060047612AG0.00015CISD1+FALSE(A)nSINE,Alu
chr255516911AG0.00719CCDC88A-FALSE(GA)nSINE,Alu
chr238971040GT0.00651SRSF7-FALSE
chr3183901281GA4.84515E-09AP2M1+FALSE
chr186852362CA0.00073ODF2L-FALSEAluSzSINE,Alu
chr10117823569GA0.00391GFRA1-FALSE(ATC)nSINE,Alu
chr178414334CA0.00702FUBP1-FALSEA-richSINE,Alu
chr1946052975TC0.00652OPA3-TRUEAluSz6SINE,Alu
chr111141187CG0.00814EXOSC10-FALSE
chr1475202532AG1.20675E-07FCF1+TRUEAluScSINE,Alu
chr127992800CA5.71645E-05IFI6-FALSEFAMSINE,Alu
chr16281866AC0.00102ICMT-FALSE
chr11597423CT2.12697E-10CDK11B,SLC35E2B-FALSE
chr132373858AT0.00100PTP4A2-FALSE
chr1226589958GA0.00613PARP1-FALSE
chr16282400TC0.00385ICMT-TRUEAluSzSINE,Alu
chr110517950GC0.00449.FALSE
chr3107526182TC7.17610E-07BBX+FALSE
chr1298942885AG1.31269E-05TMPO+TRUEAluScSINE,Alu
chr12133298917AG0.00612PGAM5+TRUEAluSgSINE,Alu
chr116928737CT0.00448NBPF1-FALSEERVL-E-intSINE,Alu
chr1144828765AT0.00976NBPF20,NBPF9+FALSE
chr2167168093CT0.00789SCN9A-FALSE
chr1226259212CA0.00167H3F3AP4,H3F3A+FALSE
chr264687777GT7.38315E-07LGALSL+FALSE
chr543380822TC0.00462CCL28-TRUEAluJbSINE,Alu
chr2217366499AG0.00183.FALSEAluSzSINE,Alu
chr642856100AG0.00048RPL7L1+TRUEAluSzSINE,Alu
chr9130268407CG0.00902FAM129B-FALSE(CTCA)nSINE,Alu
chr2203744658TC0.00112.FALSEAluSx1SINE,Alu
chr261277384AG0.00339PEX13+TRUEAluSxSINE,Alu
chr729962666TA0.00077SCRN1-FALSE
chr766007124TC0.00158GS1-124K5.11-TRUEAluJoSINE,Alu
chr286272758GA0.00023POLR1A-FALSE
chr1185014720AG0.00989RNF2+FALSE
chr1079800439AT0.00380RPS24+FALSE
chr4145031951TA0.00377GYPA-FALSE(TG)nSINE,Alu
chr1756081432GA4.92367E-05SRSF1-FALSE
chr8128944719AT0.00093PVT1+FALSE
chr4152020782CT0.00989RPS3A+FALSE
chr4248713AG0.00367ZNF876P+FALSE
chr830536450TC8.74573E-07GSR-TRUEAluSpSINE,Alu
chr629704336GA0.00207.FALSEL1ME3ASINE,Alu
chr1155853219AG0.00520SYT11+TRUEAluJbSINE,Alu
chr1075009591TC8.39163E-08DNAJC9-AS1,MRPS16+,-FALSEAluScSINE,Alu
chr23607238CT0.00222RNASEH1-AS1+FALSE
chr6114178851TC0.00056MARCKS+FALSE(TGCCGC)nSINE,Alu
chr11252662TC0.00235CPSF3L-TRUEAluSpSINE,Alu
chr124134721TC0.00113HMGCL-FALSE
chr349395694TC0.00074GPX1-FALSE
chr125894464GA0.00527LDLRAP1+FALSEMIRbSINE,Alu
chr228636644AG0.00918FOSL2+FALSE
chr143629979GT0.00470EBNA1BP2-FALSE
chr1109818162TC0.00316CELSR2+FALSE
chr3170021210CA0.00015PRKCI+FALSE
chr1109801121CT0.00115CELSR2+FALSE
chr4190883001CG0.00859FRG1+FALSE
chr6143832707GA0.00418FUCA2-FALSE(GCA)nSINE,Alu
chr11647893CT0.00509CDK11B,CDK11A-FALSE
chr631324110GA0.00521.FALSE
chr132687475TC0.00785TMEM234,EIF3I+,-FALSE
chr114599TA0.00304WASH7P-FALSE
chr140722795GA9.35494E-07.FALSECharlie2aSINE,Alu
chr1145511929AG0.00028NBPF20,NBPF10,RBM8A,GNRHR2+,-FALSEAluYSINE,Alu
chr5135513089TA7.47633E-06SMAD5+FALSE
chr128939803CT1.27013E-09TAF12-FALSE
chr2203741654TC0.00611.FALSEAluJrSINE,Alu
chr126755291AG0.00044LIN28A+TRUEAluSx1SINE,Alu
chr12125511952AG0.00138THRIL-FALSEAluSx1SINE,Alu
chr136322433GA0.00663AGO4+FALSE
chr128836872AG0.00019SNHG3,RCC1+TRUEAluScSINE,Alu
chr10102308803GC0.00324HIF1AN+FALSE(GT)nSINE,Alu
chr131905886CA0.00026SERINC2+FALSE
chr1939982631AG0.00316TIMM50+TRUEAluJrSINE,Alu
chr2242512138AG7.67847E-06BOK+FALSE
chr237331525TC0.00091.FALSEAluJbSINE,Alu
chr543175369GT7.56035E-05ZNF131+FALSE
chr332997308AG0.00082.FALSEAluYSINE,Alu
chr274090064AT0.00197STAMBP+FALSE
chr6143382538AT0.00145AIG1+FALSE
chr340503554AC0.00197RPL14+FALSE
chr193603196GA0.00627MTF2+FALSE
chr1161932324AG0.00356ATF6+FALSE
chr2209221291TG0.00020PIKFYVE+FALSEAluSxSINE,Alu
chr210908893AG0.00087ATP6V1C2+FALSE
chr193299394AG0.00655FAM69A,RPL5+,-FALSE
chr1590820083AG4.07036E-06.FALSEAluYSINE,Alu
chr543527402TC0.00539PAIP1-FALSE
chr652442880GT5.05556E-08TRAM2-AS1+FALSE
chrX77084038TC0.00265MAGT1-TRUEAluSzSINE,Alu
chr3124688639CA0.00165HEG1-FALSE
chr596372165GA0.00149.FALSE
chr439780672CA0.00743UBE2K+FALSE
chr116889502TC0.00516NBPF1-TRUEL1MC4SINE,Alu
chr1144821888CG0.00093NBPF20,NBPF9+FALSE
chr255459824CT0.00763RPS27A+FALSE
chr63022725TC0.00081HTATSF1P2-TRUEAluSzSINE,Alu
chr16585331TC0.00094NOL9-TRUEFAMSINE,Alu
chr310192652AG0.00155VHL+TRUEAluSx1SINE,Alu
chr175196073CT0.00159CRYZ-FALSE
chr5159492107AT0.00332TTC1+FALSE
chr1183105705GA0.00074LAMC1+FALSE

Differential Expression Gene Analysis