EuRBPDB

Microtus_ochrogasterFamily: Methyltr_RsmB-F Number of Genes: 6

Ensembl ID	Symbol	Entrez ID
ENSMOCG00000009613	Nsun2	102000531
ENSMOCG00000009814	Nsun6	101982305
ENSMOCG00000010041	Nsun4	101996684
ENSMOCG00000017237	Nop2	101994434
ENSMOCG00000017349	Nsun3	101986520
ENSMOCG00000021028	Nsun5	101990082

Introduction

Pfam

This is the catalytic core of this SAM-dependent 16S ribosomal methyltransferase RsmB/F enzyme [1,2]. There is a catalytic cysteine residue at 180 in UniProtKB:Q5SII2 with another highly conserved cysteine at residue 230. It methylates the C(5) position of cytosine 2870 (m5C2870) in 25S rRNA [1,2].

InterPro

The C-terminal catalytic domain of ribosomal RNA cysteine methyltransferases is highly conserved in archaeal, bacterial and eukaryotic proteins [PUBMED:21123870], such as ribosomal RNA methyltransferase B (RsmB, Sun, Fmu) and Nop2. Escherichia coli RsmB methylates cysteine C967 in 16S rRNA [PUBMED:21123870]. Nop2 methylates cysteine C2870 in the 25S rRNA of S. cerevisiae [PUBMED:23913415] and is critical for 60S biogenesis [PUBMED:8972218].

Reference

Ishikawa I, Sakai N, Tamura T, Yao M, Watanabe N, Tanaka I;, Proteins. 2004;54:814-816.: Crystal structure of human p120 homologue protein PH1374 from Pyrococcus horikoshii. PUBMED:14997580 EPMC:14997580 .
Demirci H, Larsen LH, Hansen T, Rasmussen A, Cadambi A, Gregory ST, Kirpekar F, Jogl G;, RNA. 2010;16:1584-1596.: Multi-site-specific 16S rRNA methyltransferase RsmF from Thermus thermophilus. PUBMED:20558545 EPMC:20558545.