EuRBPDB

Basic Information
Cancer Related Information

Description

RBPome

Literatures

Expression

Transcripts

Gene Model

Pathways

PPI

Orthologs

Gene Ontology

Description

Ensembl ID: ENSG00000023909 (Gene tree)
Gene ID: 2730
Gene Symbol: GCLM
Alias: GLCLR
Full Name: glutamate-cysteine ligase modifier subunit
Gene Type: protein_coding
Species: Homo_sapiens
Status: confidence
Strand: Minus strand
Length: 24,394 bases
Position: chr1:93,885,205-93,909,598
Accession: 4312
RBP type: non-canonical RBP
Summary: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

RNA binding proteome (RBPome)

PID	Title	Method	Time	Author	Doi
29431736	Capturing the interactome of newly transcribed RNA	PolyT-RICK & Hela	2018 Feb 12	Bao X	DOI: 10.1038/nmeth.4595
30528433	The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest	XRNAX & Hela	2018 Dec 6	Trendel J	DOI: 10.1016/j.cell.2018.11.004

Literatures on RNA binding capacity

PID	Title	Article	Time	Author	Doi
18279677	Glutamate cysteine ligase up-regulation fails in necrotizing pancreatitis.	Free Radic Biol Med	2008 Apr 15	Pereda J	doi: 10.1016/j.freeradbiomed.2008.01.018

Expression

Transcripts

Transcript ID	Name	Length	RefSeq ID	Protein ID	Length	RefSeq ID	UniportKB ID
ENST00000467772	GCLM-203	625	-	-	- (aa)	-	-
ENST00000615724	GCLM-204	3008	XM_011541261	ENSP00000484507	252 (aa)	XP_011539563	P48507
ENST00000370238	GCLM-201	4857	XM_017001056	ENSP00000359258	274 (aa)	XP_016856545	P48507
ENST00000462183	GCLM-202	583	-	-	- (aa)	-	-

Gene Model

Click here to download ENSG00000023909's gene model file

Pathways

Pathway ID	Pathway Name	Source
hsa00270	Cysteine and methionine metabolism	KEGG
hsa00480	Glutathione metabolism	KEGG
hsa01100	Metabolic pathways	KEGG
hsa04216	Ferroptosis	KEGG

Protein-Protein Interaction (PPI)

Clik here to download ENSG00000023909's network

* RBP PPI network refers to all genes directly bind to RBP

Orthologs identified by RBPome

Ensembl ID	Gene Symbol	Coverage	Identiy	Ortholog	Gene Symbol	Coverage	Identiy	Species
ENSG00000023909	GCLM	100	96.350	ENSMUSG00000028124	Gclm	100	96.350	Mus_musculus

Gene Ontology

Go ID	Go_term	PubmedID	Evidence	Category
GO:0004357	glutamate-cysteine ligase activity	9841880.	IDA	Function
GO:0004357	glutamate-cysteine ligase activity	16183645.	IMP	Function
GO:0005829	cytosol	9895302.	NAS	Component
GO:0005829	cytosol	-	TAS	Component
GO:0006534	cysteine metabolic process	-	IEA	Process
GO:0006536	glutamate metabolic process	9841880.9895302.	IDA	Process
GO:0006750	glutathione biosynthetic process	21873635.	IBA	Process
GO:0006750	glutathione biosynthetic process	10395918.	IDA	Process
GO:0006750	glutathione biosynthetic process	-	IEA	Process
GO:0006750	glutathione biosynthetic process	12081989.	IMP	Process
GO:0006750	glutathione biosynthetic process	-	TAS	Process
GO:0006979	response to oxidative stress	10395918.	IDA	Process
GO:0007568	aging	-	IEA	Process
GO:0007584	response to nutrient	-	IEA	Process
GO:0008637	apoptotic mitochondrial changes	-	IEA	Process
GO:0014823	response to activity	-	IEA	Process
GO:0017109	glutamate-cysteine ligase complex	21873635.	IBA	Component
GO:0017109	glutamate-cysteine ligase complex	9841880.	IDA	Component
GO:0030234	enzyme regulator activity	21873635.	IBA	Function
GO:0035226	glutamate-cysteine ligase catalytic subunit binding	9675072.9841880.	IPI	Function
GO:0035229	positive regulation of glutamate-cysteine ligase activity	21873635.	IBA	Process
GO:0035729	cellular response to hepatocyte growth factor stimulus	-	IEA	Process
GO:0035733	hepatic stellate cell activation	-	IEA	Process
GO:0042493	response to drug	9895302.	IDA	Process
GO:0043524	negative regulation of neuron apoptotic process	-	IEA	Process
GO:0044344	cellular response to fibroblast growth factor stimulus	-	IEA	Process
GO:0044752	response to human chorionic gonadotropin	-	IEA	Process
GO:0046982	protein heterodimerization activity	-	IEA	Function
GO:0050880	regulation of blood vessel size	12975258.	IMP	Process
GO:0051409	response to nitrosative stress	-	IEA	Process
GO:0051900	regulation of mitochondrial depolarization	-	IEA	Process
GO:0071333	cellular response to glucose stimulus	-	IEA	Process
GO:0071372	cellular response to follicle-stimulating hormone stimulus	-	IEA	Process
GO:0097069	cellular response to thyroxine stimulus	-	IEA	Process
GO:1990830	cellular response to leukemia inhibitory factor	-	IEA	Process
GO:2001237	negative regulation of extrinsic apoptotic signaling pathway	-	IEA	Process

Cancer associated literatures

PID	Title	Article	Time	Author	Doi
25789740	Knockdown of glutamate cysteine ligase catalytic subunit by siRNA causes the gold nanoparticles-induced cytotoxicity in lung cancer cells.	PLoS One	2015 Mar 19	Liu M	doi: 10.1371/journal.pone.0118870
14532974	The modifier subunit of glutamate cysteine ligase (GCLM) is a molecular target for amelioration of cisplatin resistance in lung cancer.	Int J Oncol	2003 Nov	Inoue Y	-
26894974	Nrf2 is the key to chemotherapy resistance in MCF7 breast cancer cells under hypoxia.	Oncotarget	2016 Mar 22	Syu JP	doi: 10.18632/oncotarget.7406.

Differential Expression

Expression in 33 cancers

Mutations

Cancer	Chr	Position	Mutation Type	dbSNP	Protein-change	Allele Freq
BLCA	chr1	93888888	3'UTR	novel		0.18
BLCA	chr1	93894701	Missense_Mutation	NA	L190F	0.36
BLCA	chr1	93904541	Silent	NA	I58I	0.19
BLCA	chr1	93897853	Missense_Mutation	novel	S108N	0.48
BRCA	chr1	93896695	Nonsense_Mutation	NA	Q155*	0.25
BRCA	chr1	93888577	3'UTR	novel		0.26
BRCA	chr1	93897881	Missense_Mutation	NA	E99Q	0.94
CESC	chr1	93901636	Missense_Mutation	NA	H76Y	0.21
COAD	chr1	93894634	Missense_Mutation	NA	L212S	0.19
COAD	chr1	93896775	Missense_Mutation	rs773867359	A128V	0.48
COAD	chr1	93904582	Missense_Mutation	novel	D45N	0.24
ESCA	chr1	93904582	Missense_Mutation	novel	D45H	0.21
ESCA	chr1	93888798	3'UTR	novel		0.47
ESCA	chr1	93888623	3'UTR	novel		0.54
GBM	chr1	93888848	3'UTR	novel		0.18
GBM	chr1	93889099	Missense_Mutation	rs761296185	A239V	0.3
HNSC	chr1	93889042	Missense_Mutation	novel	G258E	0.08
KIRC	chr1	93904544	Silent	NA	Q57Q	0.27
KIRP	chr1	93894650	Missense_Mutation	novel	F207L	0.19
LAML	chr1	93896812	Missense_Mutation	NA	V116I	0.39
LIHC	chr1	93896620	Missense_Mutation	novel	Q180E	0.46
LIHC	chr1	93889062	Silent	rs567112849	S251S	0.4
LUSC	chr1	93901612	Missense_Mutation	NA	D84Y	0.22
LUSC	chr1	93888937	3'UTR	novel		0.14
LUSC	chr1	93894669	Silent	NA	L200L	0.34
OV	chr1	93888783	3'UTR	novel		0.21
OV	chr1	93896736	Missense_Mutation	NA	E141G	0.35
PAAD	chr1	93889160	Splice_Site	novel	X219_splice	0.11
READ	chr1	93889099	Missense_Mutation	rs761296185	A239V	0.25
SKCM	chr1	93889136	Nonsense_Mutation	NA	Q227*	0.36
SKCM	chr1	93904585	Missense_Mutation	novel	H44Y	0.32
SKCM	chr1	93896748	Missense_Mutation	NA	N137I	0.4
SKCM	chr1	93889088	Missense_Mutation	rs376457310	V243M	0.67
SKCM	chr1	93896766	Missense_Mutation	novel	P131L	0.53
SKCM	chr1	93896767	Missense_Mutation	rs780522121	P131S	0.55
STAD	chr1	93894681	Missense_Mutation	NA	M196I	0.3
STAD	chr1	93889025	Missense_Mutation	NA	G264C	0.1
STAD	chr1	93894632	Missense_Mutation	NA	T213A	0.29
UCEC	chr1	93896618	Missense_Mutation	novel	Q180H	0.54
UCEC	chr1	93889045	Missense_Mutation	NA	R257K	0.36
UCEC	chr1	93901624	Missense_Mutation	novel	K80Q	0.33
UCEC	chr1	93889053	Silent	NA	V254V	0.1
UCEC	chr1	93888815	3'UTR	novel		0.29
UCEC	chr1	93894646	Missense_Mutation	rs764757939	D208G	0.36
UCEC	chr1	93889062	Silent	rs567112849	S251S	0.06
UCEC	chr1	93889000	Missense_Mutation	novel	R272M	0.13
UCEC	chr1	93888995	Missense_Mutation	novel	S274P	0.34
UCEC	chr1	93888667	3'UTR	novel		0.31
UCEC	chr1	93904567	Frame_Shift_Del	novel	*T50Pfs2**	0.31
UCEC	chr1	93889098	Silent	rs187864335	A239A	0.56
UCEC	chr1	93896635	Missense_Mutation	NA	L175M	0.35
UCEC	chr1	93896752	Missense_Mutation	novel	V136I	0.32

Copy Number Variations (CNVs)

Cancer	Type	Freq	Q-value
BRCA	DEL	0.2722	5.7821e-10
CESC	DEL	0.0814	0.01281
COAD	DEL	0.2239	0.00038973
DLBC	DEL	0.0833	0.0017831
MESO	DEL	0.4138	5.9874e-12
PRAD	DEL	0.1077	0.0061054
SKCM	DEL	0.2153	9.7877e-07
STAD	DEL	0.1746	0.0040659
TGCT	AMP	0.1733	0.088103

Survival Analysis

Cancer	P-value	Q-value
KIRC	0.00051	Kaplan-Meier Survival Analysis
SARC	0.0053	Kaplan-Meier Survival Analysis
UCS	0.041	Kaplan-Meier Survival Analysis
KIRP	0.00018	Kaplan-Meier Survival Analysis
COAD	0.026	Kaplan-Meier Survival Analysis
BLCA	0.013	Kaplan-Meier Survival Analysis
READ	0.049	Kaplan-Meier Survival Analysis
LAML	0.01	Kaplan-Meier Survival Analysis
GBM	0.03	Kaplan-Meier Survival Analysis
LIHC	0.00073	Kaplan-Meier Survival Analysis
CHOL	0.0049	Kaplan-Meier Survival Analysis
LUAD	0.035	Kaplan-Meier Survival Analysis

Drugs

EuRBPDB

Description

RBPome

Literatures

Expression

Transcripts

Gene Model

Pathways

PPI

Orthologs

Gene Ontology

Select Dataset :

Input Drug :

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Eesembl ID

Cell lines and drugs in GSE70138 or GSE92742