EuRBPDB

Basic Information
Cancer Related Information

Description

RBPome

Literatures

Expression

Transcripts

Gene Model

Pathways

PPI

Gene Ontology

Description

Ensembl ID: ENSG00000070669 (Gene tree)
Gene ID: 440
Gene Symbol: ASNS
Alias: N
Full Name: asparagine synthetase (glutamine-hydrolyzing)
Gene Type: protein_coding
Species: Homo_sapiens
Status: confidence
Strand: Minus strand
Length: 20,426 bases
Position: chr7:97,852,117-97,872,542
Accession: 753
RBP type: non-canonical RBP
Summary: The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

RNA binding proteome (RBPome)

PID	Title	Method	Time	Author	Doi
30607034	Comprehensive identification of RNA protein interactions in any organism using orthogonal organic phase separation (OOPS)	OOPS & HEK293	2019 Jan 3	Queiroz RML	DOI: 10.1038/s41587-018-0001-2
30528433	The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest	XRNAX & HEK293	2018 Dec 6	Trendel J	DOI: 10.1016/j.cell.2018.11.004
30607034	Comprehensive identification of RNA protein interactions in any organism using orthogonal organic phase separation (OOPS)	OOPS & MCF10A	2019 Jan 3	Queiroz RML	DOI: 10.1038/s41587-018-0001-2
30607034	Comprehensive identification of RNA protein interactions in any organism using orthogonal organic phase separation (OOPS)	OOPS & U2OS	2019 Jan 3	Queiroz RML	DOI: 10.1038/s41587-018-0001-2

Literatures on RNA binding capacity

PID	Title	Article	Time	Author	Doi
15385533	Amino acid deprivation induces the transcription rate of the human asparagine synthetase gene through a timed program of expression and promoter binding of nutrient-responsive basic region/leucine zipper transcription factors as well as localized histone acetylation.	J Biol Chem	2004 Dec 3	Chen H	-
18840095	Transcriptional induction of the human asparagine synthetase gene during the unfolded protein response does not require the ATF6 and IRE1/XBP1 arms of the pathway.	Biochem J	2009 Feb 1	Gjymishka A	doi: 10.1042/BJ20081706.

Expression

Transcripts

Transcript ID	Name	Length	RefSeq ID	Protein ID	Length	RefSeq ID	UniportKB ID
ENST00000453600	ASNS-212	573	-	ENSP00000408797	63 (aa)	-	C9JLN6
ENST00000437657	ASNS-207	745	-	ENSP00000394242	162 (aa)	-	C9JT45
ENST00000462436	ASNS-215	698	-	-	- (aa)	-	-
ENST00000437628	ASNS-206	1638	-	ENSP00000414379	478 (aa)	-	P08243
ENST00000414884	ASNS-204	584	-	ENSP00000413797	28 (aa)	-	C9JLA3
ENST00000448127	ASNS-210	579	-	ENSP00000402350	118 (aa)	-	C9JM09
ENST00000454046	ASNS-213	1947	-	ENSP00000401651	401 (aa)	-	F8WEJ5
ENST00000444334	ASNS-209	1812	-	ENSP00000406994	540 (aa)	-	P08243
ENST00000455086	ASNS-214	1588	-	ENSP00000408472	478 (aa)	-	P08243
ENST00000495255	ASNS-217	1678	-	-	- (aa)	-	-
ENST00000422745	ASNS-205	1865	-	ENSP00000414901	540 (aa)	-	P08243
ENST00000487714	ASNS-216	689	-	-	- (aa)	-	-
ENST00000442734	ASNS-208	828	-	ENSP00000400422	214 (aa)	-	C9J057
ENST00000175506	ASNS-201	2356	-	ENSP00000175506	561 (aa)	-	P08243
ENST00000394308	ASNS-202	1994	-	ENSP00000377845	561 (aa)	-	P08243
ENST00000394309	ASNS-203	2289	-	ENSP00000377846	561 (aa)	-	P08243
ENST00000451771	ASNS-211	562	-	ENSP00000397802	32 (aa)	-	C9J605

Gene Model

Click here to download ENSG00000070669's gene model file

Pathways

Pathway ID	Pathway Name	Source
hsa00250	Alanine, aspartate and glutamate metabolism	KEGG
hsa01100	Metabolic pathways	KEGG
hsa01230	Biosynthesis of amino acids	KEGG

Protein-Protein Interaction (PPI)

Clik here to download ENSG00000070669's network

* RBP PPI network refers to all genes directly bind to RBP

Gene Ontology

Go ID	Go_term	PubmedID	Evidence	Category
GO:0001889	liver development	-	IEA	Process
GO:0004066	asparagine synthase (glutamine-hydrolyzing) activity	21873635.	IBA	Function
GO:0004066	asparagine synthase (glutamine-hydrolyzing) activity	2564390.2569668.2573597.2886907.16023613.	IDA	Function
GO:0005515	protein binding	25416956.	IPI	Function
GO:0005524	ATP binding	-	IEA	Function
GO:0005829	cytosol	21873635.	IBA	Component
GO:0005829	cytosol	-	IDA	Component
GO:0005829	cytosol	-	TAS	Component
GO:0006529	asparagine biosynthetic process	21873635.	IBA	Process
GO:0006529	asparagine biosynthetic process	2564390.	IC	Process
GO:0006529	asparagine biosynthetic process	2573597.	IDA	Process
GO:0006541	glutamine metabolic process	-	IEA	Process
GO:0008652	cellular amino acid biosynthetic process	-	TAS	Process
GO:0009416	response to light stimulus	-	IEA	Process
GO:0009612	response to mechanical stimulus	-	IEA	Process
GO:0009636	response to toxic substance	-	IEA	Process
GO:0031427	response to methotrexate	-	IEA	Process
GO:0032354	response to follicle-stimulating hormone	-	IEA	Process
GO:0032870	cellular response to hormone stimulus	-	IEA	Process
GO:0036499	PERK-mediated unfolded protein response	-	TAS	Process
GO:0042149	cellular response to glucose starvation	10085239.	IDA	Process
GO:0042803	protein homodimerization activity	21873635.	IBA	Function
GO:0043066	negative regulation of apoptotic process	17409444.	IMP	Process
GO:0043200	response to amino acid	-	IEA	Process
GO:0045931	positive regulation of mitotic cell cycle	2569668.	IDA	Process
GO:0048037	cofactor binding	-	IEA	Function
GO:0070981	L-asparagine biosynthetic process	-	IEA	Process

Cancer associated literatures

PID	Title	Article	Time	Author	Doi
27251594	Knockdown of asparagine synthetase (ASNS) suppresses cell proliferation and inhibits tumor growth in gastric cancer cells.	Scand J Gastroenterol	2016 Oct	Yu Q	doi: 10.1080/00365521.2016.1190399
23403946	Asparagine synthetase: regulation by cell stress and involvement in tumor biology.	Am J Physiol Endocrinol Metab	2013 Apr 15	Balasubramanian MN	doi: 10.1152/ajpendo.00015.2013
20561401	[Relationship between asparagine synthetase expression level and cell sensitivity to L-asparaginase in human leukemic cell lines].	Zhongguo Shi Yan Xue Ye Xue Za Zhi	2010 Jun	Li BS	-
27444726	Loss of asparagine synthetase suppresses the growth of human lung cancer cells by arresting cell cycle at G0/G1 phase.	Cancer Gene Ther	2016 Sep	Xu Y	doi: 10.1038/cgt.2016.28
24729124	Deficiency in asparagine synthetase expression in rectal cancers receiving concurrent chemoradiotherapy: negative prognostic impact and therapeutic relevance.	Tumour Biol	2014 Jul	Lin CY	doi: 10.1007/s13277-014-1895-z
18852115	Asparagine synthetase is a predictive biomarker of L-asparaginase activity in ovarian cancer cell lines.	Mol Cancer Ther	2008 Oct	Lorenzi PL	doi: 10.1158/1535-7163.MCT-08-0589.
17088436	Asparagine synthetase as a causal, predictive biomarker for L-asparaginase activity in ovarian cancer cells.	Mol Cancer Ther	2006 Nov	Lorenzi PL	-
30061420	Inhibition of GCN2 sensitizes ASNS-low cancer cells to asparaginase by disrupting the amino acid response.	Proc Natl Acad Sci U S A	2018 Aug 14	Nakamura A	doi: 10.1073/pnas.1805523115

Differential Expression

Expression in 33 cancers

Mutations

Cancer	Chr	Position	Mutation Type	dbSNP	Protein-change	Allele Freq
BLCA	chr7	97853369	Frame_Shift_Del	novel	*P419Hfs3**	0.12
BLCA	chr7	97853167	Missense_Mutation	novel	L457M	0.05
BRCA	chr7	97854642	Missense_Mutation	novel	R392S	0.07
BRCA	chr7	97859241	Silent	NA	L215L	0.38
BRCA	chr7	97868988	Missense_Mutation	NA	F57L	0.21
BRCA	chr7	97858332	Silent	rs775400533	L283L	0.05
BRCA	chr7	97852458	Missense_Mutation	NA	A496V	0.15
BRCA	chr7	97853062	Missense_Mutation	NA	Q492K	0.16
BRCA	chr7	97858379	Missense_Mutation	novel	A268T	0.09
BRCA	chr7	97853183	Silent	rs145582437	T451T	0.06
CESC	chr7	97864394	Missense_Mutation	NA	D118H	0.05
CESC	chr7	97869165	5'UTR	novel		0.16
CESC	chr7	97869166	5'UTR	novel		0.13
CESC	chr7	97852390	Missense_Mutation	NA	R519C	0.25
CESC	chr7	97853184	Missense_Mutation	novel	T451M	0.33
CESC	chr7	97855416	Silent	rs772593615	S358S	0.2
CESC	chr7	97859268	Silent	rs775502417	H206H	0.45
CESC	chr7	97852302	Missense_Mutation	novel	S548C	0.17
COAD	chr7	97853355	Nonsense_Mutation	NA	R424*	0.13
COAD	chr7	97859376	Silent	NA	S170S	0.34
COAD	chr7	97852146	3'UTR	novel		0.32
COAD	chr7	97859254	Missense_Mutation	NA	P211L	0.18
COAD	chr7	97852239	3'UTR	novel		0.41
COAD	chr7	97859373	Silent	rs755945639	A171A	0.1
ESCA	chr7	97864357	Missense_Mutation	novel	N130S	0.54
ESCA	chr7	97852420	Missense_Mutation	novel	T509A	0.6
ESCA	chr7	97853079	Missense_Mutation	novel	Q486R	0.22
GBM	chr7	97855382	Missense_Mutation	NA	L370F	0.17
GBM	chr7	97853385	Missense_Mutation	novel	L414V	0.1
GBM	chr7	97864318	Missense_Mutation	novel	R143I	0.28
GBM	chr7	97868969	Missense_Mutation	rs761983860	R63Q	0.5
GBM	chr7	97868989	Silent	novel	L56L	0.17
HNSC	chr7	97853361	Missense_Mutation	novel	D422N	0.27
HNSC	chr7	97853079	Missense_Mutation	novel	Q486L	0.2
HNSC	chr7	97859374	Missense_Mutation	rs138299423	A171V	0.39
HNSC	chr7	97853364	Missense_Mutation	novel	L421V	0.28
HNSC	chr7	97869015	Missense_Mutation	novel	H48D	0.21
KIRP	chr7	97853335	Frame_Shift_Del	novel	*L430Ffs7**	0.22
LGG	chr7	97869123	Missense_Mutation	NA	D12H	0.17
LGG	chr7	97868976	Missense_Mutation	novel	P61S	0.24
LGG	chr7	97853138	Silent	novel	P466P	0.18
LIHC	chr7	97869160	5'UTR	NA		0.14
LIHC	chr7	97852389	Missense_Mutation	rs568570377	R519H	0.41
LIHC	chr7	97856780	Missense_Mutation	NA	L314V	0.29
LIHC	chr7	97869111	Missense_Mutation	NA	V16L	0.3
LIHC	chr7	97855398	Silent	novel	G364G	0.29
LIHC	chr7	97853200	Missense_Mutation	rs749209281	H446Y	0.19
LUAD	chr7	97852359	Missense_Mutation	NA	R529L	0.37
LUAD	chr7	97859214	Splice_Region	NA	P224P	0.16
LUAD	chr7	97852360	Missense_Mutation	rs779360130	R529W	0.04
LUAD	chr7	97854606	Silent	rs771358131	R404R	0.1
LUAD	chr7	97852352	Silent	NA	D531D	0.22
LUAD	chr7	97859273	Missense_Mutation	novel	H205D	0.06
LUAD	chr7	97855394	Missense_Mutation	novel	G366R	0.11
LUAD	chr7	97852389	Missense_Mutation	rs568570377	R519L	0.23
LUAD	chr7	97855413	Silent	novel	V359V	0.15
LUAD	chr7	97869172	5'UTR	novel		0.48
LUAD	chr7	97864390	Missense_Mutation	NA	G119V	0.67
LUAD	chr7	97852296	Missense_Mutation	NA	R550L	0.18
LUSC	chr7	97854593	Missense_Mutation	novel	T409A	0.49
LUSC	chr7	97859332	Missense_Mutation	NA	Y185C	0.47
LUSC	chr7	97856736	Missense_Mutation	NA	I328M	0.22
LUSC	chr7	97852402	Missense_Mutation	NA	G515R	0.28
LUSC	chr7	97853345	Missense_Mutation	rs753038843	S427F	0.32
LUSC	chr7	97869015	Missense_Mutation	novel	H48D	0.06
OV	chr7	97859317	Frame_Shift_Del	novel	L190*	0.09
OV	chr7	97853072	Nonsense_Mutation	NA	Y488*	0.29
OV	chr7	97858953	Missense_Mutation	novel	F226L	0.06
OV	chr7	97868999	Missense_Mutation	NA	V53A	0.22
PAAD	chr7	97856701	Missense_Mutation	novel	R340H	0.2
PAAD	chr7	97868984	Missense_Mutation	novel	G58E	0.25
PAAD	chr7	97869011	Missense_Mutation	rs769236847	R49Q	0.21
PRAD	chr7	97859292	Silent	rs527301530	S198S	0.28
READ	chr7	97858956	Splice_Site	novel	X225_splice	0.31
READ	chr7	97853117	Silent	NA	G473G	0.24
READ	chr7	97868970	Nonsense_Mutation	rs373645939	R63*	0.3
SARC	chr7	97858337	Missense_Mutation	NA	P282S	0.12
SKCM	chr7	97859293	Missense_Mutation	novel	S198F	0.43
SKCM	chr7	97869015	Missense_Mutation	novel	H48Y	0.45
SKCM	chr7	97858392	Silent	NA	S263S	0.56
SKCM	chr7	97868955	Missense_Mutation	NA	P68S	0.1
SKCM	chr7	97859369	Missense_Mutation	NA	P173S	0.64
SKCM	chr7	97856702	Missense_Mutation	novel	R340S	0.2
SKCM	chr7	97859368	Missense_Mutation	NA	P173L	0.3
SKCM	chr7	97852220	3'UTR	rs374219443		0.24
SKCM	chr7	97859345	Missense_Mutation	novel	L181F	0.39
SKCM	chr7	97854677	Missense_Mutation	rs750258058	P381S	0.36
SKCM	chr7	97858926	Missense_Mutation	NA	L235F	0.33
SKCM	chr7	97868967	Missense_Mutation	NA	V64M	0.36
SKCM	chr7	97864315	Missense_Mutation	NA	P144L	0.49
SKCM	chr7	97853195	Silent	NA	L447L	0.61
SKCM	chr7	97853097	Missense_Mutation	rs754043007	S480F	0.56
SKCM	chr7	97853134	Missense_Mutation	NA	E468K	0.44
SKCM	chr7	97858322	Missense_Mutation	novel	A287T	0.55
STAD	chr7	97864280	Missense_Mutation	NA	A156T	0.29
STAD	chr7	97854612	Silent	NA	V402V	0.19
STAD	chr7	97859373	Silent	rs755945639	A171A	0.31
STAD	chr7	97864424	Missense_Mutation	NA	G108R	0.28
STAD	chr7	97869140	Missense_Mutation	rs398122975	A6V	0.16
STAD	chr7	97858859	Frame_Shift_Del	novel	*L257Yfs15**	0.28
STAD	chr7	97852435	Nonsense_Mutation	novel	K504*	0.04
STAD	chr7	97853120	Silent	NA	D472D	0.23
STAD	chr7	97869011	Missense_Mutation	rs769236847	R49Q	0.47
UCEC	chr7	97864318	Missense_Mutation	novel	R143I	0.15
UCEC	chr7	97852397	Nonsense_Mutation	novel	Y516*	0.35
UCEC	chr7	97853348	Missense_Mutation	novel	S426F	0.05
UCEC	chr7	97868969	Missense_Mutation	rs761983860	R63Q	0.05
UCEC	chr7	97856702	Missense_Mutation	novel	R340C	0.53
UCEC	chr7	97853154	Missense_Mutation	novel	E461A	0.39
UCEC	chr7	97859375	Missense_Mutation	rs149193118	A171T	0.32
UCEC	chr7	97859382	Missense_Mutation	NA	K168N	0.26
UCEC	chr7	97853375	Missense_Mutation	NA	R417K	0.47
UCEC	chr7	97864442	Missense_Mutation	NA	L102I	0.21
UCEC	chr7	97856800	Missense_Mutation	NA	G307V	0.44
UCEC	chr7	97864362	Silent	NA	T128T	0.28
UCEC	chr7	97853354	Missense_Mutation	NA	R424Q	0.4
UCEC	chr7	97854599	Nonsense_Mutation	rs1140424	R407*	0.41
UCEC	chr7	97859374	Missense_Mutation	rs138299423	A171V	0.13
UCEC	chr7	97853071	Missense_Mutation	rs772079299	V489I	0.44
UCEC	chr7	97852434	Missense_Mutation	novel	K504R	0.25
UCEC	chr7	97858897	Missense_Mutation	novel	K244N	0.28
UCEC	chr7	97864349	Missense_Mutation	novel	V133L	0.21
UCEC	chr7	97868970	Nonsense_Mutation	rs373645939	R63*	0.44
UCEC	chr7	97859369	Missense_Mutation	novel	P173T	0.45
UCEC	chr7	97852223	3'UTR	novel		0.35
UCEC	chr7	97853058	Splice_Site	novel	X492_splice	0.26
UCEC	chr7	97856701	Missense_Mutation	novel	R340H	0.16
UCEC	chr7	97864293	Missense_Mutation	novel	E151D	0.26
UCEC	chr7	97869052	Missense_Mutation	novel	E35D	0.5
UCEC	chr7	97864304	Missense_Mutation	novel	A148S	0.59
UCEC	chr7	97852386	Missense_Mutation	novel	Q520R	0.49
UCEC	chr7	97853116	Missense_Mutation	NA	I474L	0.15
UCEC	chr7	97858355	Nonsense_Mutation	novel	E276*	0.38
UCEC	chr7	97853185	Missense_Mutation	novel	T451S	0.2
UCEC	chr7	97853351	Missense_Mutation	novel	F425Y	0.48
UCEC	chr7	97858344	Silent	novel	V279V	0.37
UCEC	chr7	97869025	Silent	novel	C44C	0.23
UCEC	chr7	97859264	Missense_Mutation	NA	R208W	0.09
UCEC	chr7	97869000	Missense_Mutation	novel	V53I	0.41
UCEC	chr7	97852451	Missense_Mutation	novel	M498I	0.34
UCS	chr7	97859375	Missense_Mutation	rs149193118	A171T	0.28

Copy Number Variations (CNVs)

Cancer	Type	Freq	Q-value
ACC	DEL	0.0556	0.12261
DLBC	AMP	0.2917	0.13215
PAAD	DEL	0.0326	0.049388
PRAD	AMP	0.1463	0.012709
TGCT	AMP	0.6533	0.035574
THCA	DEL	0.002	0.27619

Survival Analysis

Cancer	P-value	Q-value
THYM	0.026	Kaplan-Meier Survival Analysis
SARC	0.0046	Kaplan-Meier Survival Analysis
MESO	0.00034	Kaplan-Meier Survival Analysis
HNSC	0.0031	Kaplan-Meier Survival Analysis
PRAD	0.041	Kaplan-Meier Survival Analysis
LUSC	0.038	Kaplan-Meier Survival Analysis
KIRP	0.006	Kaplan-Meier Survival Analysis
LAML	0.0027	Kaplan-Meier Survival Analysis
KICH	0.0097	Kaplan-Meier Survival Analysis
UCEC	0.00055	Kaplan-Meier Survival Analysis
LIHC	0.00046	Kaplan-Meier Survival Analysis
LGG	0.0025	Kaplan-Meier Survival Analysis

Drugs

EuRBPDB

Description

RBPome

Literatures

Expression

Transcripts

Gene Model

Pathways

PPI

Gene Ontology

Select Dataset :

Input Drug :

Input Cell Line :

Eesembl ID

Cell lines and drugs in GSE70138 or GSE92742