EuRBPDB

Description

RBPome

Literatures

Expression

Transcripts

Gene Model

Pathways

Phenotypes

GWAS

PPI

Gene Ontology

Description

Ensembl ID: ENSG00000137947 (Gene tree)
Gene ID: 2959
Gene Symbol: GTF2B
Alias: TFIIB
Full Name: general transcription factor IIB
Gene Type: protein_coding
Species: Homo_sapiens
Status: confidence
Strand: Minus strand
Length: 39,307 bases
Position: chr1:88,852,638-88,891,944
Accession: 4648
RBP type: non-canonical RBP
Summary: This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008]

RNA binding proteome (RBPome)

PID	Title	Method	Time	Author	Doi
30528433	The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest	XRNAX & HEK293	2018 Dec 6	Trendel J	DOI: 10.1016/j.cell.2018.11.004
30528433	The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest	XRNAX & Hela	2018 Dec 6	Trendel J	DOI: 10.1016/j.cell.2018.11.004

Literatures on RNA binding capacity

PID	Title	Article	Time	Author	Doi
1734284	A cDNA encoding RAP74, a general initiation factor for transcription by RNA polymerase II.	Nature	1992 Jan 30	Finkelstein A	-
9237165	Yeast RNA polymerase II transcription reconstituted with purified proteins.	Methods	1997 Jul	Myers LC	-
9111063	Affinity purification of a human RNA polymerase II complex using monoclonal antibodies against transcription factor IIF.	J Biol Chem	1997 Apr 25	Cho H	-
7935466	A highly conserved domain of RNA polymerase II shares a functional element with acidic activation domains of upstream transcription factors.	Mol Cell Biol	1994 Nov	Xiao H	-
7637813	Model for binding of transcription factor TFIIB to the TBP-DNA complex.	Nature	1995 Aug 17	Lee S	-
16626286	Modulation of RNA polymerase core functions by basal transcription factor TFB/TFIIB.	Biochem Soc Symp	2006	Werner F	-
11045620	Structure of a (Cys3His) zinc ribbon, a ubiquitous motif in archaeal and eucaryal transcription.	Protein Sci	2000 Sep	Chen HT	-
10966650	The orientation of DNA in an archaeal transcription initiation complex.	Nat Struct Biol	2000 Sep	Bartlett MS	-
15916593	Archaeal transcription and its regulators.	Mol Microbiol	2005 Jun	Geiduschek EP	-
14963322	Structural basis of transcription: an RNA polymerase II-TFIIB cocrystal at 4.5 Angstroms.	Science	2004 Feb 13	Bushnell DA	-
17259543	Spliced leader RNA gene transcription in Trypanosoma brucei requires transcription factor TFIIH.	Eukaryot Cell	2007 Apr	Lee JH	-
22185463	Novel sequences of subgroup J avian leukosis viruses associated with hemangioma in Chinese layer hens.	Virol J	2011 Dec 21	Pan W	doi: 10.1186/1743-422X-8-552.
24854142	Molecular characteristics of the complete genome of a J-subgroup avian leukosis virus strain isolated from Eurasian teal in China.	Virus Genes	2014 Oct	Zeng X	doi: 10.1007/s11262-014-1081-9
1313810	Activation of RNA polymerase II transcription by the specific DNA-binding protein LSF. Increased rate of binding of the basal promoter factor TFIIB.	J Biol Chem	1992 Apr 15	Sundseth R	-
8702741	A minimal set of RNA polymerase II transcription protein interactions.	J Biol Chem	1996 Aug 16	Bushnell DA	-
10675336	A single point mutation in TFIIA suppresses NC2 requirement in vivo.	EMBO J	2000 Feb 15	Xie J	-
15385533	Amino acid deprivation induces the transcription rate of the human asparagine synthetase gene through a timed program of expression and promoter binding of nutrient-responsive basic region/leucine zipper transcription factors as well as localized histone acetylation.	J Biol Chem	2004 Dec 3	Chen H	-
14641108	Probing Zn2+-binding effects on the zinc-ribbon domain of human general transcription factor TFIIB.	Biochem J	2004 Mar 1	Ghosh M	-
14612417	The murine G+C-rich promoter binding protein mGPBP is required for promoter-specific transcription.	Mol Cell Biol	2003 Dec	Hsu LC	-
14536083	Binding of TFIIB to RNA polymerase II: Mapping the binding site for the TFIIB zinc ribbon domain within the preinitiation complex.	Mol Cell	2003 Aug	Chen HT	-
22363633	Small RNAs targeting transcription start site induce heparanase silencing through interference with transcription initiation in human cancer cells.	PLoS One	2012	Jiang G	doi: 10.1371/journal.pone.0031379
10567562	The RAP74 subunit of human transcription factor IIF has similar roles in initiation and elongation.	Mol Cell Biol	1999 Dec	Lei L	-
7556073	CCAAT/enhancer binding protein-alpha amino acid motifs with dual TBP and TFIIB binding ability co-operate to activate transcription in both yeast and mammalian cells.	EMBO J	1995 Sep 1	Nerlov C	-
7498793	The symmetry of the yeast U6 RNA gene's TATA box and the orientation of the TATA-binding protein in yeast TFIIIB.	Genes Dev	1995 Dec 1	Whitehall SK	-
3796602	Identification of two factors required for transcription of the ovalbumin gene.	Mol Cell Biol	1986 Dec	Sagami I	-
2917366	Five intermediate complexes in transcription initiation by RNA polymerase II.	Cell	1989 Feb 24	Buratowski S	-
1946368	Sequence of general transcription factor TFIIB and relationships to other initiation factors.	Proc Natl Acad Sci U S A	1991 Nov 1	Malik S	-
9689057	A post-recruitment function for the RNA polymerase III transcription-initiation factor IIIB.	Proc Natl Acad Sci U S A	1998 Aug 4	Kassavetis GA	-
9660923	Sequence-specific DNA binding by the S. shibatae TFIIB homolog, TFB, and its effect on promoter strength.	Mol Cell	1998 Feb	Qureshi SA	-
9609687	Human general transcription factor TFIIB: conformational variability and interaction with VP16 activation domain.	Biochemistry	1998 Jun 2	Hayashi F	-
9379138	The vitamin D hormone and its nuclear receptor: molecular actions and disease states.	J Endocrinol	1997 Sep	Haussler MR	-
9184236	Factor requirements for transcription in the Archaeon Sulfolobus shibatae.	EMBO J	1997 May 15	Qureshi SA	-
9121435*
%Mol Cell Biol	1997 Apr	Kumar A	-
8990171	YY1 regulates vitamin D receptor/retinoid X receptor mediated transactivation of the vitamin D responsive osteocalcin gene.	Proc Natl Acad Sci U S A	1997 Jan 7	Guo B	-
8942975	YY1 transcriptional initiator: protein interactions and association with a DNA site containing unpaired strands.	Proc Natl Acad Sci U S A	1996 Nov 26	Usheva A	-
8790357	The yeast GAL11 protein binds to the transcription factor IIE through GAL11 regions essential for its in vivo function.	Proc Natl Acad Sci U S A	1996 Sep 3	Sakurai H	-
8662660	RNA polymerase II-associated protein (RAP) 74 binds transcription factor (TF) IIB and blocks TFIIB-RAP30 binding.	J Biol Chem	1996 May 17	Fang SM	-
8662956	Cloning and functional characterization of the gene encoding the TFIIIB90 subunit of RNA polymerase III transcription factor TFIIIB.	J Biol Chem	1996 Jun 21	Roberts S	-
8621472	Localization of subunits of transcription factors IIE and IIF immediately upstream of the transcriptional initiation site of the adenovirus major late promoter.	J Biol Chem	1996 Apr 12	Robert F	-
8551580	The major transcriptional transactivation domain of simian virus 40 large T antigen associates nonconcurrently with multiple components of the transcriptional preinitiation complex.	J Virol	1996 Feb	Johnston SD	-
8490964	DNA topology and a minimal set of basal factors for transcription by RNA polymerase II.	Cell	1993 May 7	Parvin JD	-
8504927	Multiple functional domains of human transcription factor IIB: distinct interactions with two general transcription factors and RNA polymerase II.	Genes Dev	1993 Jun	Ha I	-
8515820	Functional dissection of TFIIB domains required for TFIIB-TFIID-promoter complex formation and basal transcription activity.	Nature	1993 Jun 24	Hisatake K	-
8516311	Delineation of two functional regions of transcription factor TFIIB.	Proc Natl Acad Sci U S A	1993 Jun 15	Barberis A	-
8516312	Functional domains of transcription factor TFIIB.	Proc Natl Acad Sci U S A	1993 Jun 15	Buratowski S	-
8319911	Identification of a minimal set of proteins that is sufficient for accurate initiation of transcription by RNA polymerase II.	Genes Dev	1993 Jul	Tyree CM	-
10357858	model describing transcriptional mechanisms.	Microbiol Mol Biol Rev	1999 Jun	Coulombe B	-
8137426	TATA-binding protein-independent initiation: YY1, TFIIB, and RNA polymerase II direct basal transcription on supercoiled template DNA.	Cell	1994 Mar 25	Usheva A	-
8034589	Multiple sets of basal factors initiate transcription by RNA polymerase II.	J Biol Chem	1994 Jul 15	Parvin JD	-
7995525	Conserved functional domains of the RNA polymerase III general transcription factor BRF.	Genes Dev	1994 Dec 1	Khoo B	-
7948016	Enhancement of bacterial transcription initiation in vitro by the 74 kDa subunit of human general transcription factor IIF (RAP74).	Biochim Biophys Acta	1994 Nov 22	Chibazakura T	-
7875589	Structure of the genes encoding transcription factor IIB and TATA box-binding protein from Drosophila melanogaster.	Gene	1995 Feb 14	Lira-DeVito LM	-
7823920	A spectrum of mechanisms for the assembly of the RNA polymerase II transcription preinitiation complex.	Mol Cell Biol	1995 Feb	George CP	-
12660736	Crystal structure of a transcription factor IIIB core interface ternary complex.	Nature	2003 Apr 3	Juo ZS	-
12381724	A novel archaeal transcriptional regulator of heat shock response.	J Biol Chem	2003 Jan 3	Vierke G	-
16467470	A divergent transcription factor TFIIB in trypanosomes is required for RNA polymerase II-dependent spliced leader RNA transcription and cell viability.	Eukaryot Cell	2006 Feb	Palenchar JB	-
11564744	BRFU, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with TATA-binding protein.	J Biol Chem	2001 Nov 16	Cabart P	-
10900210	Mechanism of autoregulation by an archaeal transcriptional repressor.	J Biol Chem	2000 Oct 13	Bell SD	-
10872452	Transcription elongation and human disease.	Annu Rev Biochem	1999	Conaway JW	-
10760173	The NC2 repressor is dispensable in yeast mutated for the Sin4p component of the holoenzyme and plays roles similar to Mot1p in vivo.	Mol Microbiol	2000 Apr	Lemaire M	-
16135821	Direct modulation of RNA polymerase core functions by basal transcription factors.	Mol Cell Biol	2005 Sep	Werner F	-
15652480	Mechanism of transcriptional silencing in yeast.	Cell	2005 Jan 14	Chen L	-
14729958	Photo-cross-linking of a purified preinitiation complex reveals central roles for the RNA polymerase II mobile clamp and TFIIE in initiation mechanisms.	Mol Cell Biol	2004 Feb	Forget D	-
14645237	Developmentally specific role of the CCAAT box in regulation of human gamma-globin gene expression.	J Biol Chem	2004 Feb 13	Fang X	-
19818408	Improved methods for expression and purification of Saccharomyces cerevisiae TFIIF and TFIIH; identification of a functional Escherichia coli promoter and internal translation initiation within the N-terminal coding region of the TFIIF TFG1 subunit.	Protein Expr Purif	2010 Apr	Yang C	doi: 10.1016/j.pep.2009.09.021
19590095	Minimal promoter systems reveal the importance of conserved residues in the B-finger of human transcription factor IIB.	J Biol Chem	2009 Sep 11	Thompson NE	doi: 10.1074/jbc.M109.030486
22999857	Chromatin immunoprecipitation and microarray analysis reveal that TFIIB occupies the SL RNA gene promoter region in Trypanosoma brucei chromosomes.	Mol Biochem Parasitol	2012 Dec	Liu W	doi: 10.1016/j.molbiopara.2012.09.003
18235446	The X-ray crystal structure of RNA polymerase from Archaea.	Nature	2008 Feb 14	Hirata A	doi: 10.1038/nature06530
17275836	TFB1 or TFB2 is sufficient for Thermococcus kodakaraensis viability and for basal transcription in vitro.	J Mol Biol	2007 Mar 23	Santangelo TJ	-
22424775	Dynamic architecture of a minimal RNA polymerase II open promoter complex.	Mol Cell	2012 Apr 27	Treutlein B	doi: 10.1016/j.molcel.2012.02.008
22365827	Conservation between the RNA polymerase I, II, and III transcription initiation machineries.	Mol Cell	2012 Feb 24	Vannini A	doi: 10.1016/j.molcel.2012.01.023.
22025674	Architecture of the yeast RNA polymerase II open complex and regulation of activity by TFIIF.	Mol Cell Biol	2012 Jan	Fishburn J	doi: 10.1128/MCB.06242-11
21835917	Evidence for a complex of transcription factor IIB with poly(A) polymerase and cleavage factor 1 subunits required for gene looping.	J Biol Chem	2011 Sep 30	Medler S	doi: 10.1074/jbc.M110.193870
21468301	Molecular architecture of the human Mediator-RNA polymerase II-TFIIF assembly.	PLoS Biol	2011 Mar	Bernecky C	doi: 10.1371/journal.pbio.1000603
23176534	Class II-specific histone deacetylase inhibitors MC1568 and MC1575 suppress IL-8 expression in human melanoma cells.	Pigment Cell Melanoma Res	2013 Mar	Venza I	doi: 10.1111/pcmr.12049
23151482	Structure and function of the initially transcribing RNA polymerase II-TFIIB complex.	Nature	2013 Jan 17	Sainsbury S	doi: 10.1038/nature11715
24048476	Genomic organization of human transcription initiation complexes.	Nature	2013 Oct 3	Venters BJ	doi: 10.1038/nature12535
26284261	In vitro fluorescence studies of transcription factor IIB-DNA interaction.	Acta Biochim Pol	2015	Gorecki A	doi: 10.18388/abp.2015_1034

Expression

Transcripts

Transcript ID	Name	Length	RefSeq ID	Protein ID	Length	RefSeq ID	UniportKB ID
ENST00000370500	GTF2B-201	1351	XM_011541299	ENSP00000359531	316 (aa)	XP_011539601	Q00403
ENST00000471296	GTF2B-204	593	-	-	- (aa)	-	-
ENST00000448623	GTF2B-203	770	-	ENSP00000415741	224 (aa)	-	B1APE1
ENST00000486078	GTF2B-206	528	-	-	- (aa)	-	-
ENST00000494819	GTF2B-207	893	-	-	- (aa)	-	-
ENST00000418217	GTF2B-202	783	-	ENSP00000402345	231 (aa)	-	B1APE2
ENST00000471471	GTF2B-205	516	-	-	- (aa)	-	-

Gene Model

Click here to download ENSG00000137947's gene model file

Pathways

Pathway ID	Pathway Name	Source
hsa03022	Basal transcription factors	KEGG
hsa05203	Viral carcinogenesis	KEGG

Phenotypes

ensgID	Trait	pValue	Pubmed ID
ENSG00000137947	Intercellular Adhesion Molecule-1	4E-6	23251661

GWAS

ensgID	SNP	Chromosome	Position	SNP-risk	Trait	PubmedID	95% CI	Or or BEAT	EFO ID
ENSG00000137947	rs10127863	1	88878573	G	Intraocular pressure	29617998	[0.055-0.11] unit increase	0.0826	EFO_0004695
ENSG00000137947	rs17130643	1	88875725	A	Obesity-related traits	23251661	[NR] pg/mL increase	0.02	EFO_0004520
ENSG00000137947	rs12740480	1	88886929	?	Waist-hip ratio	30595370			EFO_0004343
ENSG00000137947	rs12035750	1	88866416	?	Systolic blood pressure	30595370			EFO_0006335
ENSG00000137947	rs12035750	1	88866416	C	Systolic blood pressure	30578418	[0.14-0.28] mmHg decrease	0.2125	EFO_0006335

Protein-Protein Interaction (PPI)

Clik here to download ENSG00000137947's network

* RBP PPI network refers to all genes directly bind to RBP

Gene Ontology

Go ID	Go_term	PubmedID	Evidence	Category
GO:0000979	RNA polymerase II core promoter sequence-specific DNA binding	21873635.	IBA	Function
GO:0000979	RNA polymerase II core promoter sequence-specific DNA binding	9420329.16230532.	IDA	Function
GO:0000993	RNA polymerase II complex binding	8413225.8504927.	IDA	Function
GO:0001047	core promoter binding	7675079.10619841.	IDA	Function
GO:0001139	RNA polymerase II complex recruiting activity	21873635.	IBA	Function
GO:0001174	transcriptional start site selection at RNA polymerase II promoter	21873635.	IBA	Process
GO:0001174	transcriptional start site selection at RNA polymerase II promoter	10318856.	IMP	Process
GO:0004402	histone acetyltransferase activity	-	IEA	Function
GO:0005515	protein binding	1517211.8006019.8415761.8504927.8662660.10330164.10449590.10619841.12529369.12552007.12931194.14644612.15207618.15207693.25416956.29158257.	IPI	Function
GO:0005634	nucleus	21873635.	IBA	Component
GO:0005634	nucleus	9841876.12931194.	IDA	Component
GO:0005654	nucleoplasm	-	TAS	Component
GO:0005669	transcription factor TFIID complex	27193682.	IDA	Component
GO:0005694	chromosome	24441171.	IDA	Component
GO:0006352	DNA-templated transcription, initiation	21873635.	IBA	Process
GO:0006366	transcription by RNA polymerase II	27193682.	IDA	Process
GO:0006366	transcription by RNA polymerase II	1517211.1876184.7601352.8413225.8515820.8516312.16230532.	IMP	Process
GO:0006366	transcription by RNA polymerase II	-	TAS	Process
GO:0006367	transcription initiation from RNA polymerase II promoter	3029109.	IDA	Process
GO:0006367	transcription initiation from RNA polymerase II promoter	-	TAS	Process
GO:0006473	protein acetylation	12931194.	IDA	Process
GO:0008134	transcription factor binding	21873635.	IBA	Function
GO:0008134	transcription factor binding	9722567.	IPI	Function
GO:0008270	zinc ion binding	11045620.	IDA	Function
GO:0016032	viral process	-	IEA	Process
GO:0016251	RNA polymerase II general transcription initiation factor activity	27193682.	IDA	Function
GO:0016407	acetyltransferase activity	12931194.	IDA	Function
GO:0016573	histone acetylation	-	IEA	Process
GO:0016604	nuclear body	-	IDA	Component
GO:0017025	TBP-class protein binding	21873635.	IBA	Function
GO:0017025	TBP-class protein binding	9841876.	IPI	Function
GO:0032993	protein-DNA complex	8413225.8515820.8516312.10619841.29158257.	IDA	Component
GO:0042795	snRNA transcription by RNA polymerase II	-	TAS	Process
GO:0043923	positive regulation by host of viral transcription	24441171.	IDA	Process
GO:0046966	thyroid hormone receptor binding	8524305.	IPI	Function
GO:0050434	positive regulation of viral transcription	14644612.	IMP	Process
GO:0051123	RNA polymerase II preinitiation complex assembly	21873635.	IBA	Process
GO:0051123	RNA polymerase II preinitiation complex assembly	3029109.9841876.	IDA	Process
GO:0051123	RNA polymerase II preinitiation complex assembly	8413225.	IMP	Process
GO:0060261	positive regulation of transcription initiation from RNA polymerase II promoter	9420329.12931194.	IMP	Process
GO:0090575	RNA polymerase II transcription factor complex	1876184.3029109.	IDA	Component
GO:0090575	RNA polymerase II transcription factor complex	7601352.	IMP	Component
GO:0097550	transcriptional preinitiation complex	21873635.	IBA	Component
GO:0097550	transcriptional preinitiation complex	9841876.	IDA	Component
GO:1904798	positive regulation of core promoter binding	9841876.	IDA	Process
GO:1990114	RNA polymerase II core complex assembly	8515820.8516312.	IMP	Process
GO:1990841	promoter-specific chromatin binding	12931194.24441171.	IDA	Function