EuRBPDB

Description

RBDs

RBPome

Literatures

Expression

Transcripts

Gene Model

Pathways

GWAS

PPI

Orthologs

Gene Ontology

Description

Ensembl ID: ENSG00000158417 (Gene tree)
Gene ID: 9669
Gene Symbol: EIF5B
Alias: IF2|KIAA0741|DKFZp434I036|FLJ10524
Full Name: eukaryotic translation initiation factor 5B
Gene Type: protein_coding
Species: Homo_sapiens
Status: confidence
Strand: Plus strand
Length: 63,974 bases
Position: chr2:99,337,353-99,401,326
Accession: 30793
RBP type: canonical RBP
Summary: Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq, Jul 2008]

RNA binding domains(RBDs)

Protein ID	Domain	Pfam ID	E-value	Domain number	Total number
ENSP00000289371	IF-2	PF11987.8	1.4e-18	1	1
ENSP00000478914	IF-2	PF11987.8	1.4e-18	1	1
ENSP00000289371	GTP_EFTU	PF00009.27	3.1e-34	1	1
ENSP00000478914	GTP_EFTU	PF00009.27	3.1e-34	1	1
ENSP00000289371	MMR_HSR1	PF01926.23	2.9e-05	1	1
ENSP00000478914	MMR_HSR1	PF01926.23	2.9e-05	1	1
ENSP00000289371	GTP_EFTU_D2	PF03144.25	5.1e-09	1	1
ENSP00000478914	GTP_EFTU_D2	PF03144.25	5.2e-09	1	1

RNA binding proteome (RBPome)

PID	Title	Method	Time	Author	Doi
22681889	The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts	4SURIC & HEK293	2012 May	Baltz AG	DOI: 10.1016/j.molcel.2012.05.021
30607034	Comprehensive identification of RNA protein interactions in any organism using orthogonal organic phase separation (OOPS)	OOPS & HEK293	2019 Jan 3	Queiroz RML	DOI: 10.1038/s41587-018-0001-2
30528433	The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest	XRNAX & HEK293	2018 Dec 6	Trendel J	DOI: 10.1016/j.cell.2018.11.004
29431736	Capturing the interactome of newly transcribed RNA	PolyT-RICK & Hela	2018 Feb 12	Bao X	DOI: 10.1038/nmeth.4595
29431736	Capturing the interactome of newly transcribed RNA	RIC & Hela	2018 Feb 12	Bao X	DOI: 10.1038/nmeth.4595
22658674	Insights into RNA biology from an atlas of mammalian mRNA-binding proteins	RIC & Hela	2012 May 31	Castello A	DOI: 10.1016/j.cell.2012.04.031
30528433	The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest	XRNAX & Hela	2018 Dec 6	Trendel J	DOI: 10.1016/j.cell.2018.11.004
30607034	Comprehensive identification of RNA protein interactions in any organism using orthogonal organic phase separation (OOPS)	OOPS & MCF10A	2019 Jan 3	Queiroz RML	DOI: 10.1038/s41587-018-0001-2
30528433	The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest	XRNAX & MCF7	2018 Dec 6	Trendel J	DOI: 10.1016/j.cell.2018.11.004
30607034	Comprehensive identification of RNA protein interactions in any organism using orthogonal organic phase separation (OOPS)	OOPS & U2OS	2019 Jan 3	Queiroz RML	DOI: 10.1038/s41587-018-0001-2
27453046	Comprehensive Identification of RNA-Binding Domains in Human Cells	RIC & Hela	2016 Aug 18	Castello A	DOI: 10.1016/j.molcel.2016.06.029

Literatures on RNA binding capacity

PID	Title	Article	Time	Author	Doi
23766293	Two RNA-binding motifs in eIF3 direct HCV IRES-dependent translation.	Nucleic Acids Res	2013 Aug	Sun C	doi: 10.1093/nar/gkt510
27364543	A model for the interaction of the G3-subdomain of Geobacillus stearothermophilus IF2 with the 30S ribosomal subunit.	Protein Sci	2016 Sep	Dongre R	doi: 10.1002/pro.2977
25512054	Genes associated with thermosensitive genic male sterility in rice identified by comparative expression profiling.	BMC Genomics	2014 Dec 16	Pan Y	doi: 10.1186/1471-2164-15-1114.
12643896	Inhibition of bacterial IF2 binding to fMet-tRNA((fMet)) by aminoglycosides.	Bioorg Med Chem Lett	2003 Mar 24	Evans JM	-
1764105	Tandem translation of E. coli initiation factor IF2 beta: purification and characterization in vitro of two active forms.	Biochem Biophys Res Commun	1991 Dec 31	Nyengaard NR	-
22723375	Translation initiation without IF2-dependent GTP hydrolysis.	Nucleic Acids Res	2012 Sep	Fabbretti A	doi: 10.1093/nar/gks569
23150791	GTPases IF2 and EF-G bind GDP and the SRL RNA in a mutually exclusive manner.	Sci Rep	2012	Mitkevich VA	doi: 10.1038/srep00843

Expression

Transcripts

Transcript ID	Name	Length	RefSeq ID	Protein ID	Length	RefSeq ID	UniportKB ID
ENST00000494190	EIF5B-204	664	-	-	- (aa)	-	-
ENST00000289371	EIF5B-201	5777	-	ENSP00000289371	1220 (aa)	-	O60841
ENST00000617677	EIF5B-205	4698	-	ENSP00000478914	1220 (aa)	-	A0A087WUT6
ENST00000470868	EIF5B-203	573	-	-	- (aa)	-	-
ENST00000470023	EIF5B-202	2331	-	-	- (aa)	-	-

Gene Model

Click here to download ENSG00000158417's gene model file

Pathways

Pathway ID	Pathway Name	Source
hsa03013	RNA transport	KEGG

GWAS

ensgID	SNP	Chromosome	Position	SNP-risk	Trait	PubmedID	95% CI	Or or BEAT	EFO ID
ENSG00000158417	rs62155770	2	99364759	A	Educational attainment (MTAG)	30038396	[0.011-0.023] unit decrease	0.0173	EFO_0004784
ENSG00000158417	rs115028891	2	99346967	?	General cognitive ability	29844566	z-score decrease	4.895	EFO_0004337
ENSG00000158417	rs62155770	2	99364759	A	Educational attainment (years of education)	30038396	[0.013-0.026] unit decrease	0.0196	EFO_0004784

Protein-Protein Interaction (PPI)

Clik here to download ENSG00000158417's network

* RBP PPI network refers to all genes directly bind to RBP

Orthologs

Ensembl ID	Gene Symbol	Coverage	Identiy	Ortholog	Gene Symbol	Coverage	Identiy	Species
ENSG00000158417	EIF5B	100	94.845	ENSAMEG00000013269	EIF5B	100	95.008	Ailuropoda_melanoleuca
ENSG00000158417	EIF5B	99	83.047	ENSAPLG00000015401	EIF5B	100	83.620	Anas_platyrhynchos
ENSG00000158417	EIF5B	100	98.526	ENSANAG00000017300	EIF5B	100	98.526	Aotus_nancymaae
ENSG00000158417	EIF5B	100	92.875	ENSBTAG00000034255	EIF5B	99	93.476	Bos_taurus
ENSG00000158417	EIF5B	100	95.172	ENSCAFG00000002278	EIF5B	100	95.336	Canis_familiaris
ENSG00000158417	EIF5B	98	94.561	ENSCAFG00020024623	EIF5B	99	93.445	Canis_lupus_dingo
ENSG00000158417	EIF5B	100	93.781	ENSCHIG00000027106	EIF5B	100	93.781	Capra_hircus
ENSG00000158417	EIF5B	99	95.289	ENSTSYG00000011850	EIF5B	100	95.455	Carlito_syrichta
ENSG00000158417	EIF5B	99	97.858	ENSCCAG00000027156	EIF5B	99	97.858	Cebus_capucinus
ENSG00000158417	EIF5B	100	98.853	ENSCATG00000038292	EIF5B	100	98.853	Cercocebus_atys
ENSG00000158417	EIF5B	100	91.728	ENSCLAG00000007674	EIF5B	100	92.138	Chinchilla_lanigera
ENSG00000158417	EIF5B	100	98.773	ENSCSAG00000017209	EIF5B	100	98.773	Chlorocebus_sabaeus
ENSG00000158417	EIF5B	100	83.306	ENSCPBG00000005318	EIF5B	100	83.387	Chrysemys_picta_bellii
ENSG00000158417	EIF5B	100	98.771	ENSCANG00000042984	EIF5B	100	98.771	Colobus_angolensis_palliatus
ENSG00000158417	EIF5B	100	92.471	ENSCGRG00001010937	Eif5b	100	92.308	Cricetulus_griseus_chok1gshd
ENSG00000158417	EIF5B	99	92.473	ENSCGRG00000003140	Eif5b	100	92.308	Cricetulus_griseus_crigri
ENSG00000158417	EIF5B	94	93.543	ENSDNOG00000005016	EIF5B	99	93.194	Dasypus_novemcinctus
ENSG00000158417	EIF5B	100	90.008	ENSETEG00000001467	EIF5B	100	89.762	Echinops_telfairi
ENSG00000158417	EIF5B	100	94.267	ENSEASG00005018269	EIF5B	100	95.004	Equus_asinus_asinus
ENSG00000158417	EIF5B	100	95.250	ENSECAG00000000102	EIF5B	100	95.414	Equus_caballus
ENSG00000158417	EIF5B	99	94.248	ENSFCAG00000009782	EIF5B	100	94.412	Felis_catus
ENSG00000158417	EIF5B	100	92.635	ENSFDAG00000008737	EIF5B	100	93.044	Fukomys_damarensis
ENSG00000158417	EIF5B	100	83.266	ENSGALG00000016764	EIF5B	100	83.659	Gallus_gallus
ENSG00000158417	EIF5B	100	99.590	ENSGGOG00000001721	EIF5B	100	99.590	Gorilla_gorilla
ENSG00000158417	EIF5B	100	91.810	ENSHGLG00000016367	EIF5B	100	91.810	Heterocephalus_glaber_female
ENSG00000158417	EIF5B	100	91.892	ENSHGLG00100016185	EIF5B	100	91.892	Heterocephalus_glaber_male
ENSG00000158417	EIF5B	100	94.922	ENSSTOG00000014283	EIF5B	100	95.004	Ictidomys_tridecemlineatus
ENSG00000158417	EIF5B	100	68.421	ENSLOCG00000001405	EIF5B	51	87.299	Lepisosteus_oculatus
ENSG00000158417	EIF5B	100	93.535	ENSLAFG00000006209	EIF5B	100	91.817	Loxodonta_africana
ENSG00000158417	EIF5B	100	98.853	ENSMFAG00000038108	EIF5B	100	98.853	Macaca_fascicularis
ENSG00000158417	EIF5B	100	98.935	ENSMMUG00000018946	EIF5B	100	98.935	Macaca_mulatta
ENSG00000158417	EIF5B	100	98.771	ENSMNEG00000040038	EIF5B	100	98.771	Macaca_nemestrina
ENSG00000158417	EIF5B	99	98.760	ENSMLEG00000031596	EIF5B	100	98.760	Mandrillus_leucophaeus
ENSG00000158417	EIF5B	99	82.630	ENSMGAG00000014793	EIF5B	100	82.792	Meleagris_gallopavo
ENSG00000158417	EIF5B	99	96.777	ENSMICG00000015960	EIF5B	99	96.777	Microcebus_murinus
ENSG00000158417	EIF5B	100	89.853	MGP_CAROLIEiJ_G0014061	Eif5b	100	89.689	Mus_caroli
ENSG00000158417	EIF5B	100	90.180	ENSMUSG00000026083	Eif5b	100	90.016	Mus_musculus
ENSG00000158417	EIF5B	100	89.444	MGP_PahariEiJ_G0027308	Eif5b	100	89.607	Mus_pahari
ENSG00000158417	EIF5B	100	90.098	MGP_SPRETEiJ_G0014865	Eif5b	100	89.935	Mus_spretus
ENSG00000158417	EIF5B	100	94.758	ENSMPUG00000012068	EIF5B	100	94.676	Mustela_putorius_furo
ENSG00000158417	EIF5B	99	93.064	ENSMLUG00000008119	EIF5B	100	92.733	Myotis_lucifugus
ENSG00000158417	EIF5B	100	99.098	ENSNLEG00000014172	EIF5B	100	99.098	Nomascus_leucogenys
ENSG00000158417	EIF5B	93	88.850	ENSOPRG00000007359	EIF5B	93	89.025	Ochotona_princeps
ENSG00000158417	EIF5B	99	95.410	ENSOGAG00000013238	EIF5B	99	96.040	Otolemur_garnettii
ENSG00000158417	EIF5B	100	93.776	ENSOARG00000013400	EIF5B	100	93.776	Ovis_aries
ENSG00000158417	EIF5B	99	96.777	ENSPPAG00000029857	EIF5B	100	96.777	Pan_paniscus
ENSG00000158417	EIF5B	99	95.706	ENSPPRG00000010820	EIF5B	99	95.706	Panthera_pardus
ENSG00000158417	EIF5B	99	92.857	ENSPTIG00000021215	EIF5B	99	93.021	Panthera_tigris_altaica
ENSG00000158417	EIF5B	100	99.672	ENSPTRG00000012279	EIF5B	100	99.672	Pan_troglodytes
ENSG00000158417	EIF5B	100	98.608	ENSPANG00000021269	EIF5B	100	98.608	Papio_anubis
ENSG00000158417	EIF5B	99	84.653	ENSPCIG00000011603	EIF5B	99	85.136	Phascolarctos_cinereus
ENSG00000158417	EIF5B	84	98.333	ENSPPYG00000012094	EIF5B	100	98.333	Pongo_abelii
ENSG00000158417	EIF5B	99	88.522	ENSRNOG00000023356	Eif5b	100	88.357	Rattus_norvegicus
ENSG00000158417	EIF5B	100	88.380	ENSRNOG00000046621	AABR07043748.1	100	88.216	Rattus_norvegicus
ENSG00000158417	EIF5B	99	96.614	ENSRBIG00000028180	EIF5B	99	96.614	Rhinopithecus_bieti
ENSG00000158417	EIF5B	100	98.853	ENSRROG00000038801	EIF5B	100	98.853	Rhinopithecus_roxellana
ENSG00000158417	EIF5B	100	97.954	ENSSBOG00000033578	EIF5B	100	97.954	Saimiri_boliviensis_boliviensis
ENSG00000158417	EIF5B	100	86.765	ENSSHAG00000010146	EIF5B	100	87.010	Sarcophilus_harrisii
ENSG00000158417	EIF5B	99	92.479	ENSSARG00000002096	EIF5B	100	92.645	Sorex_araneus
ENSG00000158417	EIF5B	99	81.034	ENSSPUG00000009445	EIF5B	99	80.870	Sphenodon_punctatus
ENSG00000158417	EIF5B	100	94.431	ENSSSCG00000008180	EIF5B	100	94.595	Sus_scrofa
ENSG00000158417	EIF5B	100	81.316	ENSTGUG00000010099	EIF5B	100	81.848	Taeniopygia_guttata
ENSG00000158417	EIF5B	100	92.547	ENSTTRG00000010374	EIF5B	100	92.711	Tursiops_truncatus
ENSG00000158417	EIF5B	99	94.715	ENSUMAG00000008519	EIF5B	95	99.081	Ursus_maritimus
ENSG00000158417	EIF5B	88	93.290	ENSVPAG00000007725	EIF5B	100	93.476	Vicugna_pacos
ENSG00000158417	EIF5B	100	95.254	ENSVVUG00000028702	EIF5B	100	95.417	Vulpes_vulpes

Gene Ontology

Go ID	Go_term	PubmedID	Evidence	Category
GO:0003723	RNA binding	22658674.22681889.	HDA	Function
GO:0003743	translation initiation factor activity	21873635.	IBA	Function
GO:0003743	translation initiation factor activity	10432305.	IDA	Function
GO:0003743	translation initiation factor activity	10200264.	NAS	Function
GO:0003924	GTPase activity	-	IEA	Function
GO:0005515	protein binding	12569173.20195357.	IPI	Function
GO:0005525	GTP binding	-	IEA	Function
GO:0005634	nucleus	12426392.	IDA	Component
GO:0005737	cytoplasm	12426392.	IDA	Component
GO:0005829	cytosol	-	TAS	Component
GO:0006413	translational initiation	-	IEA	Process
GO:0006446	regulation of translational initiation	10432305.	IDA	Process
GO:0006446	regulation of translational initiation	10200264.	NAS	Process
GO:0046872	metal ion binding	-	IEA	Function