EuRBPDB

Description

Ensembl ID: ENSG00000167881 (Gene tree)
Gene ID: 6730
Gene Symbol: SRP68
Alias: N
Full Name: signal recognition particle 68
Gene Type: protein_coding
Species: Homo_sapiens
Status: confidence
Strand: Minus strand
Length: 33,879 bases
Position: chr17:76,038,775-76,072,653
Accession: 11302
RBP type: non-canonical RBP
Summary: This gene encodes a subunit of the signal recognition particle (SRP). The SRP is a ribonucleoprotein complex that transports secreted and membrane proteins to the endoplasmic reticulum for processing. The complex includes a 7S RNA and six protein subunits. The encoded protein is the 68kDa component of the SRP, and forms a heterodimer with the 72kDa subunit that is required for SRP function. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and three pseudogenes of this gene are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, May 2012]

RNA binding proteome (RBPome)

PID	Title	Method	Time	Author	Doi
30528433	The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest	XRNAX & HEK293	2018 Dec 6	Trendel J	DOI: 10.1016/j.cell.2018.11.004
29431736	Capturing the interactome of newly transcribed RNA	PolyT-RICK & Hela	2018 Feb 12	Bao X	DOI: 10.1038/nmeth.4595
29431736	Capturing the interactome of newly transcribed RNA	RIC & Hela	2018 Feb 12	Bao X	DOI: 10.1038/nmeth.4595
22658674	Insights into RNA biology from an atlas of mammalian mRNA-binding proteins	RIC & Hela	2012 May 31	Castello A	DOI: 10.1016/j.cell.2012.04.031
30528433	The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest	XRNAX & Hela	2018 Dec 6	Trendel J	DOI: 10.1016/j.cell.2018.11.004
30607034	Comprehensive identification of RNA protein interactions in any organism using orthogonal organic phase separation (OOPS)	OOPS & MCF10A	2019 Jan 3	Queiroz RML	DOI: 10.1038/s41587-018-0001-2
30528433	The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest	XRNAX & MCF7	2018 Dec 6	Trendel J	DOI: 10.1016/j.cell.2018.11.004
30607034	Comprehensive identification of RNA protein interactions in any organism using orthogonal organic phase separation (OOPS)	OOPS & U2OS	2019 Jan 3	Queiroz RML	DOI: 10.1038/s41587-018-0001-2

Literatures on RNA binding capacity

PID	Title	Article	Time	Author	Doi
1377027	Fluorescence-detected assembly of the signal recognition particle: binding of the two SRP protein heterodimers to SRP RNA is noncooperative.	Biochemistry	1992 Jun 30	Janiak F	-
18347438	Identification of the RNA binding regions of SRP68/72 and SRP72 by systematic mutagenesis of human SRP RNA.	RNA Biol	2007 Nov	Yin J	-
24700861	SRP RNA remodeling by SRP68 explains its role in protein translocation.	Science	2014 Apr 4	Grotwinkel JT	doi: 10.1126/science.1249094.
1702390	The 68 kDa protein of signal recognition particle contains a glycine-rich region also found in certain RNA-binding proteins.	FEBS Lett	1990 Dec 10	Herz J	-
16672232	Protein SRP68 of human signal recognition particle: identification of the RNA and SRP72 binding domains.	Protein Sci	2006 Jun	Iakhiaeva E	-
7518075	Molecular evolution of SRP cycle components: functional implications.	Nucleic Acids Res	1994 Jun 11	Althoff S	-
18564060	Anti-cooperative assembly of the SRP19 and SRP68/72 components of the signal recognition particle.	Biochem J	2008 Nov 1	Maity TS	doi: 10.1042/BJ20080569.
17254600	Protein-induced conformational changes of RNA during the assembly of human signal recognition particle.	J Mol Biol	2007 Mar 16	Menichelli E	-

Expression

Transcripts

Transcript ID	Name	Length	RefSeq ID	Protein ID	Length	RefSeq ID	UniportKB ID
ENST00000586859	SRP68-205	703	-	-	- (aa)	-	-
ENST00000592704	SRP68-211	1941	-	ENSP00000466791	54 (aa)	-	K7EN53
ENST00000590833	SRP68-209	582	-	-	- (aa)	-	-
ENST00000588643	SRP68-207	2040	-	-	- (aa)	-	-
ENST00000591272	SRP68-210	507	-	ENSP00000467765	63 (aa)	-	K7EQC2
ENST00000602720	SRP68-212	2037	-	ENSP00000473613	288 (aa)	-	Q9UHB9
ENST00000629930	SRP68-213	165	-	ENSP00000486633	54 (aa)	-	K7EN53
ENST00000307877	SRP68-201	2649	-	ENSP00000312066	627 (aa)	-	Q9UHB9
ENST00000542536	SRP68-203	1714	-	-	- (aa)	-	-
ENST00000587864	SRP68-206	577	-	-	- (aa)	-	-
ENST00000588736	SRP68-208	2532	-	-	- (aa)	-	-
ENST00000585539	SRP68-204	254	-	-	- (aa)	-	-
ENST00000539137	SRP68-202	2386	-	ENSP00000446136	589 (aa)	-	Q9UHB9

Gene Model

Pathways

Pathway ID	Pathway Name	Source
hsa03060	Protein export	KEGG

Protein-Protein Interaction (PPI)

* RBP PPI network refers to all genes directly bind to RBP

Orthologs identified by RBPome

Ensembl ID	Gene Symbol	Coverage	Identiy	Ortholog	Gene Symbol	Coverage	Identiy	Species
ENSG00000167881	SRP68	88	32.297	WBGene00010097	srpa-68	89	32.815	Caenorhabditis_elegans
ENSG00000167881	SRP68	98	39.236	FBgn0035947	Srp68	94	42.440	Drosophila_melanogaster
ENSG00000167881	SRP68	100	94.896	ENSMUSG00000020780	Srp68	100	94.577	Mus_musculus

Gene Ontology

Go ID	Go_term	PubmedID	Evidence	Category
GO:0003723	RNA binding	22658674.	HDA	Function
GO:0005047	signal recognition particle binding	21873635.	IBA	Function
GO:0005047	signal recognition particle binding	17254600.	IPI	Function
GO:0005515	protein binding	24700861.24965446.	IPI	Function
GO:0005730	nucleolus	-	IEA	Component
GO:0005783	endoplasmic reticulum	10618370.	TAS	Component
GO:0005786	signal recognition particle, endoplasmic reticulum targeting	21873635.	IBA	Component
GO:0005786	signal recognition particle, endoplasmic reticulum targeting	18089836.	IDA	Component
GO:0005829	cytosol	-	IDA	Component
GO:0005829	cytosol	-	TAS	Component
GO:0005840	ribosome	10618370.	TAS	Component
GO:0005925	focal adhesion	-	IDA	Component
GO:0006614	SRP-dependent cotranslational protein targeting to membrane	-	TAS	Process
GO:0008312	7S RNA binding	27899666.	IMP	Function
GO:0019904	protein domain specific binding	27899666.	IPI	Function
GO:0030942	endoplasmic reticulum signal peptide binding	-	IEA	Function
GO:0042493	response to drug	18089836.	IDA	Process
GO:0043022	ribosome binding	27899666.	IMP	Function
GO:0048500	signal recognition particle	27899666.	IDA	Component