EuRBPDB

Description

Ensembl ID: ENSG00000169598 (Gene tree)
Gene ID: 1677
Gene Symbol: DFFB
Alias: CAD|CPAN|DFF-40|DFF40
Full Name: DNA fragmentation factor subunit beta
Gene Type: protein_coding
Species: Homo_sapiens
Status: putative
Strand: Plus strand
Length: 28,287 bases
Position: chr1:3,857,143-3,885,429
Accession: 2773
RBP type: non-canonical RBP
Summary: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. [provided by RefSeq, Sep 2013]

RNA binding proteome (RBPome)

PID	Title	Method	Time	Author	Doi
30607034	Comprehensive identification of RNA protein interactions in any organism using orthogonal organic phase separation (OOPS)	OOPS & HEK293	2019 Jan 3	Queiroz RML	DOI: 10.1038/s41587-018-0001-2
30528433	The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest	XRNAX & HEK293	2018 Dec 6	Trendel J	DOI: 10.1016/j.cell.2018.11.004
29431736	Capturing the interactome of newly transcribed RNA	PolyT-RICK & Hela	2018 Feb 12	Bao X	DOI: 10.1038/nmeth.4595
30607034	Comprehensive identification of RNA protein interactions in any organism using orthogonal organic phase separation (OOPS)	OOPS & MCF10A	2019 Jan 3	Queiroz RML	DOI: 10.1038/s41587-018-0001-2

Expression

Transcripts

Transcript ID	Name	Length	RefSeq ID	Protein ID	Length	RefSeq ID	UniportKB ID
ENST00000477548	DFFB-209	2923	-	ENSP00000462175	103 (aa)	-	G3XAD0
ENST00000378206	DFFB-204	2842	-	ENSP00000367448	80 (aa)	-	Q96P72
ENST00000491998	DFFB-211	3152	-	ENSP00000436775	270 (aa)	-	O76075
ENST00000468793	DFFB-207	2986	-	ENSP00000463688	115 (aa)	-	Q96P73
ENST00000378209	DFFB-205	3028	XM_017000498	ENSP00000367454	338 (aa)	XP_016855987	O76075
ENST00000475969	DFFB-208	2731	-	ENSP00000463704	115 (aa)	-	Q96P73
ENST00000339350	DFFB-202	3005	-	ENSP00000343218	116 (aa)	-	O76075
ENST00000461150	DFFB-206	686	-	ENSP00000462770	78 (aa)	-	J3KT26
ENST00000338895	DFFB-201	3105	XM_017000499	ENSP00000339524	362 (aa)	XP_016855988	B4DZS0
ENST00000481945	DFFB-210	636	-	-	- (aa)	-	-
ENST00000625756	DFFB-212	649	-	ENSP00000486916	103 (aa)	-	G3XAD0
ENST00000341385	DFFB-203	572	-	ENSP00000345906	82 (aa)	-	A0A0B4J1S5

Gene Model

Pathways

Pathway ID	Pathway Name	Source
hsa04210	Apoptosis	KEGG

Protein-Protein Interaction (PPI)

* RBP PPI network refers to all genes directly bind to RBP

Orthologs identified by RBPome

Ensembl ID	Gene Symbol	Coverage	Identiy	Ortholog	Gene Symbol	Coverage	Identiy	Species
ENSG00000169598	DFFB	100	77.219	ENSMUSG00000029027	Dffb	98	77.219	Mus_musculus

Gene Ontology

Go ID	Go_term	PubmedID	Evidence	Category
GO:0000790	nuclear chromatin	21873635.	IBA	Component
GO:0000790	nuclear chromatin	19882353.	IDA	Component
GO:0000790	nuclear chromatin	22253444.	IDA	Component
GO:0004536	deoxyribonuclease activity	21873635.	IBA	Function
GO:0004536	deoxyribonuclease activity	19944011.	IMP	Function
GO:0005515	protein binding	19882353.	IPI	Function
GO:0005634	nucleus	10959840.15572351.22253444.	IDA	Component
GO:0005654	nucleoplasm	-	TAS	Component
GO:0005730	nucleolus	-	IDA	Component
GO:0005829	cytosol	9108473.22253444.	IDA	Component
GO:0005829	cytosol	-	TAS	Component
GO:0006308	DNA catabolic process	19944011.	IMP	Process
GO:0006309	apoptotic DNA fragmentation	21873635.	IBA	Process
GO:0006309	apoptotic DNA fragmentation	15572351.22253444.	IDA	Process
GO:0006309	apoptotic DNA fragmentation	-	TAS	Process
GO:0019899	enzyme binding	10959840.	IPI	Function
GO:0019904	protein domain specific binding	19944011.	IPI	Function
GO:0030263	apoptotic chromosome condensation	10959840.	IDA	Process
GO:0042802	identical protein binding	11371636.	IMP	Function
GO:0051260	protein homooligomerization	11371636.	IMP	Process
GO:0097718	disordered domain specific binding	11371636.	IPI	Function