EuRBPDB

Description

RBDs

RBPome

Expression

Transcripts

Gene Model

PPI

Paralogs

Orthologs

Gene Ontology

Description

Ensembl ID: ENSMUSG00000028820 (Gene tree)
Gene ID: 71514
Gene Symbol: Sfpq
Alias: 1110004P21Rik|2810416M14Rik|5730453G22Rik|9030402K04Rik|D4Ertd314e|PSF|REP1
Full Name: splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated)
Gene Type: protein_coding
Species: Mus_musculus
Status: confidence
Strand: Plus strand
Length: 15690 bases
Position: chr4:127021324-127037013
Accession: 1918764
RBP type: canonical RBP
Summary: -

RNA binding domains(RBDs)

Protein ID	Domain	Pfam ID	E-value	Domain number	Total number
ENSMUSP00000030623	RRM_1	PF00076.22	7.6e-30	1	2
ENSMUSP00000030623	RRM_1	PF00076.22	7.6e-30	2	2

RNA binding proteome (RBPome)

PID	Title	Method	Time	Author	Doi
25993413	Label-Free Protein RNA Interactome Analysis Identifies Khsrp Signaling Downstream of the p38/Mk2 Kinase Complex as a Critical Modulator of Cell Cycle Progression	IC & MEF	2015	Boucas J	DOI: 10.1371/journal.pone.0125745
27768875	High Resolution Mapping of RNA Binding Regions in the Nuclear Proteome of Embryonic Stem Cells	nIC & mESC	2016.09	He C	DOI: 10.1016/j.molcel.2016.09.034
27281784	Identification of RNA binding Proteins in Macrophages by Interactome Capture	IC & RAW264.7	2016 Jun 8	Liepelt A	DOI: 10.1074/mcp.M115.056564
23912277	The RNA binding protein repertoire of embryonic stem cells	IC & mESC	2013 Aug 4	Kwon SC	DOI: 10.1038/nsmb.2638
27452465	The Cardiomyocyte RNA Binding Proteome: Links to Intermediary Metabolism and Heart Disease	IC & HL_1	2016 Jul 21	Liao Y	DOI: 10.1016/j.celrep.2016.06.084
27452465	The Cardiomyocyte RNA Binding Proteome: Links to Intermediary Metabolism and Heart Disease	RBDmap & HL_1	2016 Jul 21	Liao Y	DOI: 10.1016/j.celrep.2016.06.084

Expression

Transcripts

Transcript ID	Name	Length	RefSeq ID	Protein ID	Length	RefSeq ID	UniportKB ID
ENSMUST00000140682	-	755	-	-	- (aa)	-	-
ENSMUST00000030623	-	3465	XM_006503406	ENSMUSP00000030623	699 (aa)	XP_006503469	Q8VIJ6
ENSMUST00000125453	-	644	-	-	- (aa)	-	-
ENSMUST00000144630	-	600	-	-	- (aa)	-	-
ENSMUST00000146285	-	925	NR_045010	-	- (aa)	-	-
ENSMUST00000143168	-	967	-	-	- (aa)	-	-

Gene Model

Click here to download ENSMUSG00000028820's gene model file

Protein-Protein Interaction (PPI)

Clik here to download ENSMUSG00000028820's network

Paralogs

Ensembl ID	Gene Symbol	Coverage	Identiy	Paralog	Gene Symbol	Coverage	Identiy
ENSMUSG00000028820	Sfpq	55	65.891	ENSMUSG00000021938	Pspc1	83	71.429

Orthologs

Ensembl ID	Gene Symbol	Coverage	Identiy	Ortholog	Gene Symbol	Coverage	Identiy	Species
ENSMUSG00000028820	Sfpq	69	98.347	ENSG00000116560	SFPQ	97	99.074	Homo_sapiens
ENSMUSG00000028820	Sfpq	62	74.596	ENSAPOG00000024257	sfpq	62	75.000	Acanthochromis_polyacanthus
ENSMUSG00000028820	Sfpq	63	99.318	ENSAMEG00000000815	SFPQ	100	99.318	Ailuropoda_melanoleuca
ENSMUSG00000028820	Sfpq	80	61.348	ENSACIG00000000905	sfpq	78	65.618	Amphilophus_citrinellus
ENSMUSG00000028820	Sfpq	62	74.246	ENSAOCG00000002889	sfpq	60	74.312	Amphiprion_ocellaris
ENSMUSG00000028820	Sfpq	62	74.246	ENSAPEG00000016556	sfpq	60	74.312	Amphiprion_percula
ENSMUSG00000028820	Sfpq	66	73.763	ENSATEG00000002981	sfpq	67	76.082	Anabas_testudineus
ENSMUSG00000028820	Sfpq	50	76.000	ENSANAG00000037798	-	99	75.714	Aotus_nancymaae
ENSMUSG00000028820	Sfpq	50	75.141	ENSANAG00000021268	-	100	74.074	Aotus_nancymaae
ENSMUSG00000028820	Sfpq	92	96.154	ENSANAG00000027438	-	68	82.766	Aotus_nancymaae
ENSMUSG00000028820	Sfpq	63	69.977	ENSAMXG00000011538	sfpq	65	72.500	Astyanax_mexicanus
ENSMUSG00000028820	Sfpq	69	98.347	ENSBTAG00000016328	SFPQ	68	98.954	Bos_taurus
ENSMUSG00000028820	Sfpq	69	98.140	ENSCJAG00000001431	SFPQ	68	98.745	Callithrix_jacchus
ENSMUSG00000028820	Sfpq	83	95.697	ENSCAFG00000003595	-	73	98.745	Canis_familiaris
ENSMUSG00000028820	Sfpq	85	84.333	ENSCAFG00000018271	-	66	92.744	Canis_familiaris
ENSMUSG00000028820	Sfpq	85	81.135	ENSCAFG00000017608	-	65	92.517	Canis_familiaris
ENSMUSG00000028820	Sfpq	85	84.193	ENSCAFG00020004881	-	66	92.744	Canis_lupus_dingo
ENSMUSG00000028820	Sfpq	50	90.598	ENSCAFG00020010702	-	100	90.598	Canis_lupus_dingo
ENSMUSG00000028820	Sfpq	69	98.347	ENSCHIG00000023097	SFPQ	67	98.954	Capra_hircus
ENSMUSG00000028820	Sfpq	50	85.100	ENSTSYG00000035106	-	98	85.100	Carlito_syrichta
ENSMUSG00000028820	Sfpq	50	99.430	ENSTSYG00000037120	-	100	99.430	Carlito_syrichta
ENSMUSG00000028820	Sfpq	50	90.883	ENSCPOG00000015018	-	100	88.981	Cavia_porcellus
ENSMUSG00000028820	Sfpq	69	97.934	ENSCCAG00000029361	-	68	98.536	Cebus_capucinus
ENSMUSG00000028820	Sfpq	61	94.811	ENSCATG00000036179	-	61	95.923	Cercocebus_atys
ENSMUSG00000028820	Sfpq	69	72.301	ENSCATG00000032664	-	66	72.934	Cercocebus_atys
ENSMUSG00000028820	Sfpq	50	99.430	ENSCLAG00000009583	-	100	99.430	Chinchilla_lanigera
ENSMUSG00000028820	Sfpq	69	98.347	ENSCSAG00000001082	SFPQ	68	98.954	Chlorocebus_sabaeus
ENSMUSG00000028820	Sfpq	54	94.737	ENSCHOG00000009131	SFPQ	100	94.737	Choloepus_hoffmanni
ENSMUSG00000028820	Sfpq	63	93.213	ENSCPBG00000018674	SFPQ	69	92.568	Chrysemys_picta_bellii
ENSMUSG00000028820	Sfpq	50	99.430	ENSCANG00000028087	-	100	99.430	Colobus_angolensis_palliatus
ENSMUSG00000028820	Sfpq	100	97.866	ENSCGRG00001011698	Sfpq	100	97.866	Cricetulus_griseus_chok1gshd
ENSMUSG00000028820	Sfpq	62	100.000	ENSCGRG00000010128	Sfpq	95	100.000	Cricetulus_griseus_crigri
ENSMUSG00000028820	Sfpq	62	74.600	ENSCSEG00000007852	sfpq	66	73.165	Cynoglossus_semilaevis
ENSMUSG00000028820	Sfpq	61	70.023	ENSCVAG00000019538	sfpq	64	67.371	Cyprinodon_variegatus
ENSMUSG00000028820	Sfpq	63	71.526	ENSDARG00000011564	sfpq	100	83.478	Danio_rerio
ENSMUSG00000028820	Sfpq	69	97.934	ENSDNOG00000049454	SFPQ	84	95.142	Dasypus_novemcinctus
ENSMUSG00000028820	Sfpq	69	98.347	ENSDORG00000008109	Sfpq	85	95.980	Dipodomys_ordii
ENSMUSG00000028820	Sfpq	62	92.575	ENSETEG00000001095	SFPQ	61	92.575	Echinops_telfairi
ENSMUSG00000028820	Sfpq	62	72.917	ENSELUG00000023145	sfpq	66	71.694	Esox_lucius
ENSMUSG00000028820	Sfpq	85	95.645	ENSFCAG00000042862	SFPQ	85	95.645	Felis_catus
ENSMUSG00000028820	Sfpq	50	94.587	ENSFALG00000000731	SFPQ	100	94.587	Ficedula_albicollis
ENSMUSG00000028820	Sfpq	50	90.313	ENSFDAG00000017723	-	100	90.652	Fukomys_damarensis
ENSMUSG00000028820	Sfpq	62	67.126	ENSFHEG00000015200	sfpq	56	68.736	Fundulus_heteroclitus
ENSMUSG00000028820	Sfpq	68	68.399	ENSGMOG00000011957	sfpq	65	73.782	Gadus_morhua
ENSMUSG00000028820	Sfpq	62	74.943	ENSGMOG00000011950	SFPQ	74	75.000	Gadus_morhua
ENSMUSG00000028820	Sfpq	63	94.292	ENSGALG00000002514	SFPQ	68	94.292	Gallus_gallus
ENSMUSG00000028820	Sfpq	90	53.251	ENSGAFG00000014571	sfpq	57	77.607	Gambusia_affinis
ENSMUSG00000028820	Sfpq	80	62.248	ENSGACG00000002034	sfpq	68	74.654	Gasterosteus_aculeatus
ENSMUSG00000028820	Sfpq	75	99.076	ENSGGOG00000026354	SFPQ	100	99.430	Gorilla_gorilla
ENSMUSG00000028820	Sfpq	68	98.129	ENSHGLG00000012001	SFPQ	86	91.500	Heterocephalus_glaber_female
ENSMUSG00000028820	Sfpq	50	99.430	ENSHGLG00100004136	-	100	99.430	Heterocephalus_glaber_male
ENSMUSG00000028820	Sfpq	64	72.991	ENSHCOG00000008612	sfpq	70	68.372	Hippocampus_comes
ENSMUSG00000028820	Sfpq	65	69.248	ENSIPUG00000013076	SFPQ	73	72.540	Ictalurus_punctatus
ENSMUSG00000028820	Sfpq	50	99.430	ENSSTOG00000014004	-	100	99.430	Ictidomys_tridecemlineatus
ENSMUSG00000028820	Sfpq	69	98.554	ENSJJAG00000015633	Sfpq	68	98.745	Jaculus_jaculus
ENSMUSG00000028820	Sfpq	62	69.725	ENSKMAG00000000238	sfpq	54	71.034	Kryptolebias_marmoratus
ENSMUSG00000028820	Sfpq	62	76.256	ENSLACG00000014728	SFPQ	70	80.324	Latimeria_chalumnae
ENSMUSG00000028820	Sfpq	90	62.096	ENSLOCG00000001067	sfpq	68	81.912	Lepisosteus_oculatus
ENSMUSG00000028820	Sfpq	97	87.001	ENSLAFG00000015951	SFPQ	85	95.142	Loxodonta_africana
ENSMUSG00000028820	Sfpq	69	98.140	ENSMFAG00000000797	SFPQ	68	98.745	Macaca_fascicularis
ENSMUSG00000028820	Sfpq	69	98.140	ENSMMUG00000019154	SFPQ	68	98.745	Macaca_mulatta
ENSMUSG00000028820	Sfpq	69	98.347	ENSMNEG00000038895	SFPQ	68	98.954	Macaca_nemestrina
ENSMUSG00000028820	Sfpq	50	96.296	ENSMLEG00000010643	-	100	96.296	Mandrillus_leucophaeus
ENSMUSG00000028820	Sfpq	78	66.727	ENSMAMG00000007875	sfpq	66	77.726	Mastacembelus_armatus
ENSMUSG00000028820	Sfpq	54	91.049	ENSMGAG00000004237	SFPQ	100	91.049	Meleagris_gallopavo
ENSMUSG00000028820	Sfpq	100	97.866	ENSMAUG00000006402	Sfpq	100	97.866	Mesocricetus_auratus
ENSMUSG00000028820	Sfpq	69	98.347	ENSMICG00000027265	SFPQ	68	98.954	Microcebus_murinus
ENSMUSG00000028820	Sfpq	69	95.652	ENSMOCG00000019075	Sfpq	100	95.652	Microtus_ochrogaster
ENSMUSG00000028820	Sfpq	80	64.134	ENSMMOG00000001074	sfpq	65	74.651	Mola_mola
ENSMUSG00000028820	Sfpq	64	95.291	ENSMODG00000017937	SFPQ	72	93.595	Monodelphis_domestica
ENSMUSG00000028820	Sfpq	62	75.116	ENSMALG00000010381	sfpq	64	75.584	Monopterus_albus
ENSMUSG00000028820	Sfpq	100	99.857	MGP_CAROLIEiJ_G0026487	Sfpq	100	99.857	Mus_caroli
ENSMUSG00000028820	Sfpq	100	99.142	MGP_PahariEiJ_G0028818	Sfpq	100	99.142	Mus_pahari
ENSMUSG00000028820	Sfpq	100	100.000	MGP_SPRETEiJ_G0027466	Sfpq	100	100.000	Mus_spretus
ENSMUSG00000028820	Sfpq	64	96.444	ENSMLUG00000027090	SFPQ	100	96.444	Myotis_lucifugus
ENSMUSG00000028820	Sfpq	69	98.557	ENSNGAG00000000888	Sfpq	68	98.956	Nannospalax_galili
ENSMUSG00000028820	Sfpq	90	57.845	ENSNBRG00000017479	sfpq	64	73.077	Neolamprologus_brichardi
ENSMUSG00000028820	Sfpq	50	99.430	ENSNLEG00000035521	-	100	99.430	Nomascus_leucogenys
ENSMUSG00000028820	Sfpq	66	95.900	ENSMEUG00000014082	SFPQ	63	95.238	Notamacropus_eugenii
ENSMUSG00000028820	Sfpq	73	93.294	ENSOPRG00000013696	SFPQ	68	96.234	Ochotona_princeps
ENSMUSG00000028820	Sfpq	66	87.013	ENSODEG00000005485	SFPQ	85	86.422	Octodon_degus
ENSMUSG00000028820	Sfpq	63	73.423	ENSONIG00000017737	sfpq	57	79.781	Oreochromis_niloticus
ENSMUSG00000028820	Sfpq	50	96.866	ENSOANG00000008789	-	100	96.866	Ornithorhynchus_anatinus
ENSMUSG00000028820	Sfpq	73	89.443	ENSOCUG00000001877	SFPQ	92	94.167	Oryctolagus_cuniculus
ENSMUSG00000028820	Sfpq	72	65.029	ENSORLG00000007459	sfpq	57	71.694	Oryzias_latipes
ENSMUSG00000028820	Sfpq	82	56.825	ENSORLG00020011818	sfpq	57	71.694	Oryzias_latipes_hni
ENSMUSG00000028820	Sfpq	72	64.775	ENSORLG00015002131	sfpq	58	71.034	Oryzias_latipes_hsok
ENSMUSG00000028820	Sfpq	99	52.493	ENSOMEG00000018020	sfpq	58	70.295	Oryzias_melastigma
ENSMUSG00000028820	Sfpq	76	96.597	ENSOGAG00000027710	SFPQ	100	96.597	Otolemur_garnettii
ENSMUSG00000028820	Sfpq	74	99.306	ENSOARG00000019439	SFPQ	80	99.306	Ovis_aries
ENSMUSG00000028820	Sfpq	50	99.148	ENSPPRG00000015478	-	100	99.148	Panthera_pardus
ENSMUSG00000028820	Sfpq	50	99.430	ENSPTIG00000007710	-	100	99.430	Panthera_tigris_altaica
ENSMUSG00000028820	Sfpq	69	92.149	ENSPTRG00000000518	SFPQ	66	92.678	Pan_troglodytes
ENSMUSG00000028820	Sfpq	78	66.117	ENSPKIG00000023035	sfpq	73	79.493	Paramormyrops_kingsleyae
ENSMUSG00000028820	Sfpq	62	94.664	ENSPSIG00000011494	SFPQ	100	94.664	Pelodiscus_sinensis
ENSMUSG00000028820	Sfpq	61	69.249	ENSPMGG00000023883	sfpq	58	81.424	Periophthalmus_magnuspinnatus
ENSMUSG00000028820	Sfpq	67	99.151	ENSPEMG00000009794	Sfpq	97	99.151	Peromyscus_maniculatus_bairdii
ENSMUSG00000028820	Sfpq	64	95.516	ENSPCIG00000000923	SFPQ	69	93.595	Phascolarctos_cinereus
ENSMUSG00000028820	Sfpq	86	55.803	ENSPFOG00000003127	sfpq	71	68.360	Poecilia_formosa
ENSMUSG00000028820	Sfpq	69	98.347	ENSPPYG00000001560	SFPQ	68	98.954	Pongo_abelii
ENSMUSG00000028820	Sfpq	77	98.368	ENSPCAG00000006164	SFPQ	77	98.368	Procavia_capensis
ENSMUSG00000028820	Sfpq	69	98.347	ENSPCOG00000027127	SFPQ	68	98.954	Propithecus_coquereli
ENSMUSG00000028820	Sfpq	96	92.136	ENSPVAG00000001865	SFPQ	85	93.132	Pteropus_vampyrus
ENSMUSG00000028820	Sfpq	63	71.396	ENSPNYG00000007494	sfpq	62	72.624	Pundamilia_nyererei
ENSMUSG00000028820	Sfpq	87	58.497	ENSPNAG00000002128	sfpq	73	72.973	Pygocentrus_nattereri
ENSMUSG00000028820	Sfpq	100	97.282	ENSRNOG00000058461	Sfpq	100	97.282	Rattus_norvegicus
ENSMUSG00000028820	Sfpq	99	72.103	ENSRBIG00000035286	SFPQ	100	99.430	Rhinopithecus_bieti
ENSMUSG00000028820	Sfpq	68	100.000	ENSRROG00000040992	SFPQ	100	99.430	Rhinopithecus_roxellana
ENSMUSG00000028820	Sfpq	50	99.430	ENSSBOG00000024972	-	100	99.430	Saimiri_boliviensis_boliviensis
ENSMUSG00000028820	Sfpq	50	91.453	ENSSHAG00000012312	-	100	90.883	Sarcophilus_harrisii
ENSMUSG00000028820	Sfpq	62	90.869	ENSSHAG00000016107	-	97	90.200	Sarcophilus_harrisii
ENSMUSG00000028820	Sfpq	75	66.231	ENSSFOG00015009289	sfpq	79	77.778	Scleropages_formosus
ENSMUSG00000028820	Sfpq	62	77.083	ENSSMAG00000018201	sfpq	66	73.982	Scophthalmus_maximus
ENSMUSG00000028820	Sfpq	62	76.620	ENSSDUG00000008402	sfpq	65	78.291	Seriola_dumerili
ENSMUSG00000028820	Sfpq	62	76.620	ENSSLDG00000018917	sfpq	65	78.291	Seriola_lalandi_dorsalis
ENSMUSG00000028820	Sfpq	53	99.457	ENSSARG00000006409	SFPQ	100	99.457	Sorex_araneus
ENSMUSG00000028820	Sfpq	85	94.807	ENSSSCG00000035690	SFPQ	96	90.370	Sus_scrofa
ENSMUSG00000028820	Sfpq	62	94.896	ENSTGUG00000001722	SFPQ	100	94.896	Taeniopygia_guttata
ENSMUSG00000028820	Sfpq	62	75.402	ENSTRUG00000003783	sfpq	62	76.092	Takifugu_rubripes
ENSMUSG00000028820	Sfpq	63	76.484	ENSTNIG00000011331	sfpq	72	76.027	Tetraodon_nigroviridis
ENSMUSG00000028820	Sfpq	69	87.009	ENSTBEG00000006238	SFPQ	79	72.673	Tupaia_belangeri
ENSMUSG00000028820	Sfpq	85	90.787	ENSTTRG00000013930	SFPQ	85	91.792	Tursiops_truncatus
ENSMUSG00000028820	Sfpq	50	98.374	ENSUAMG00000026758	SFPQ	85	98.374	Ursus_americanus
ENSMUSG00000028820	Sfpq	50	96.581	ENSUMAG00000008056	-	100	96.581	Ursus_maritimus
ENSMUSG00000028820	Sfpq	64	88.964	ENSVPAG00000006960	-	67	88.964	Vicugna_pacos
ENSMUSG00000028820	Sfpq	50	88.319	ENSVVUG00000000874	-	100	88.319	Vulpes_vulpes
ENSMUSG00000028820	Sfpq	65	79.957	ENSXETG00000009682	sfpq	100	84.830	Xenopus_tropicalis

Gene Ontology

Go ID	Go_term	PubmedID	Evidence	Category
GO:0000122	negative regulation of transcription by RNA polymerase II	-	ISO	Process
GO:0000380	alternative mRNA splicing, via spliceosome	21873635.	IBA	Process
GO:0000380	alternative mRNA splicing, via spliceosome	-	ISO	Process
GO:0000724	double-strand break repair via homologous recombination	20813759.	ISO	Process
GO:0000785	chromatin	-	ISO	Component
GO:0000976	transcription regulatory region sequence-specific DNA binding	21873635.	IBA	Function
GO:0000976	transcription regulatory region sequence-specific DNA binding	21680841.	IDA	Function
GO:0000980	RNA polymerase II distal enhancer sequence-specific DNA binding	19389484.	IDA	Function
GO:0002218	activation of innate immune response	-	ISO	Process
GO:0002376	immune system process	-	IEA	Process
GO:0003676	nucleic acid binding	-	IEA	Function
GO:0003677	DNA binding	-	ISO	Function
GO:0003682	chromatin binding	19389484.	IDA	Function
GO:0003723	RNA binding	-	IEA	Function
GO:0005515	protein binding	18495661.19144721.20813759.21177649.21680841.22966205.23055941.23308148.23863478.	IPI	Function
GO:0005634	nucleus	21873635.	IBA	Component
GO:0005634	nucleus	-	ISO	Component
GO:0005654	nucleoplasm	-	ISO	Component
GO:0005737	cytoplasm	-	IEA	Component
GO:0006281	DNA repair	-	IEA	Process
GO:0006397	mRNA processing	-	IEA	Process
GO:0006974	cellular response to DNA damage stimulus	20813759.	IMP	Process
GO:0008380	RNA splicing	-	IEA	Process
GO:0016363	nuclear matrix	-	ISO	Component
GO:0042382	paraspeckles	21444682.	IDA	Component
GO:0042382	paraspeckles	-	ISO	Component
GO:0042752	regulation of circadian rhythm	22966205.	IMP	Process
GO:0042754	negative regulation of circadian rhythm	21680841.	IMP	Process
GO:0042803	protein homodimerization activity	-	ISO	Function
GO:0042826	histone deacetylase binding	-	ISO	Function
GO:0044212	transcription regulatory region DNA binding	-	ISO	Function
GO:0045087	innate immune response	-	IEA	Process
GO:0045876	positive regulation of sister chromatid cohesion	20813759.	IMP	Process
GO:0045892	negative regulation of transcription, DNA-templated	22966205.	IDA	Process
GO:0045892	negative regulation of transcription, DNA-templated	21680841.	IMP	Process
GO:0045944	positive regulation of transcription by RNA polymerase II	-	ISO	Process
GO:0048511	rhythmic process	-	IEA	Process
GO:0051276	chromosome organization	20813759.	IMP	Process
GO:0051726	regulation of cell cycle	20813759.	IMP	Process
GO:0070888	E-box binding	22966205.	IDA	Function
GO:0070932	histone H3 deacetylation	21680841.	IMP	Process
GO:0090575	RNA polymerase II transcription factor complex	19389484.	IDA	Component
GO:0098963	dendritic transport of messenger ribonucleoprotein complex	15312650.	IDA	Process
GO:0098963	dendritic transport of messenger ribonucleoprotein complex	15312650.	IMP	Process
GO:1902177	positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway	-	ISO	Process