Mus_musculusFamily: tRNA_int_endo_N Number of Genes: 1
Ensembl IDSymbolEntrez IDRBD RBPome PRIExpresion PathwayPhenotype ParalogOrthologGO



Members of this family cleave pre tRNA at the 5' and 3' splice sites to release the intron EC:


This entry represents a 2-layer alpha/beta domain found at the N terminus of the homotetrameric tRNA-intron endonucleases [PUBMED:9535656], and as domains 1 (N-terminal) and 3 in the homodimeric enzymes [PUBMED:16690865]. tRNA-intron endonucleases (EC) remove tRNA introns by cleaving pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-hydroxyl termini [PUBMED:9200602]. These enzymes recognise a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp [PUBMED:14993668]. Although homotetrameric enzymes contain four active sites, only two participate in the cleavage, and should therefore, be considered as a dimer of dimers.


  1. Kleman-Leyer K, Armbruster DW, Daniels CJ; , Cell 1997;89:839-847.: Properties of H. volcanii tRNA intron endonuclease reveal a relationship between the archaeal and eucaryal tRNA intron processing systems. PUBMED:9200602 EPMC:9200602 .

  2. Li H, Trotta CR, Abelson J; , Science 1998;280:279-284.: Crystal structure and evolution of a transfer RNA splicing enzyme. PUBMED:9535656 EPMC:9535656.